牛肉中三种β-受体激动剂的质谱检测方法  

Determination of 3 Typesβ-Receptor Agonist in Beef by Mass Spectrometry

作　　者：张睿泓 张成成 张炳珏 王成艳 马永顺 王昕泽 杜光光 李海霞 ZHANG Ruihong;ZHANG Chengcheng;ZHANG Bingjue;WANG Chengyan;MA Yongshun;WANG Xinze;DU Guangguang;LI Haixia(Changchun Product Quality Supervision and Inspection Institute,Changchun 130000,China;Hangzhou Expec Technology Development Corporation,Hangzhou 310000,China)

机构地区：[1]长春市产品质量监督检验院,长春130000 [2]杭州谱育科技发展有限公司,杭州310000

出　　处：《草食家畜》2024年第1期47-51,共5页Grass-Feeding Livestock

摘　　要：【目的】在确保结果准确度,精密度合格的情况下,对现行牛肉中莱克多巴胺、沙丁胺醇和克伦特罗的残留含量检测方法进行优化,以降低操作难度,提高检测效率。【方法】样品经0.1%乙酸乙腈溶液提取,水饱和正己烷去脂,提取溶液经氮吹仪浓缩,复溶后溶液采用HPLC-MS/MS检测分析,内标法定量。【结果】本方法在1.0~50.0 ng/mL线性范围响应良好,三种加标水平下,莱克多巴胺、克伦特罗和沙丁胺醇的回收率分别为94.3%~107.9%,92.2%~105.7%和92.2%~102.3%,定量检测限均低于0.06μg/kg,精密度均不超过10%。【结论】本方法具有较低的定量检测限,步骤简单的样品处理过程,高准确度和良好的稳定性,可满足大批量牛肉样品(含有以上三种待测物质)的痕量检测需求。【Objective】To optimize the current residual detection methods for ractopamine,salbutamol,and clenbuterol in beef to reduce operational difficulties and improve the detection efficiency under the condition of ensuring the accuracy of the results and qualified precision.【Methods】The sample was extracted by 0.1%acetonitrile acetate solution,and defatted by water saturated nhexane,The extraction solution was concentrated by nitrogen blowing instrument,the re-solubilised solution was detected and analyzed by HPLC-MS/MS,and quantified by internal standard method.【Results】The linear range of this method was 1.0~50.0 ng/mL with good response.The recoveries of ractopamine,clenbuterol and salbutamol at the three spiked levels were 94.3%~107.9%,92.2%~105.7%and 92.2%~102.3%,respectively,and the limits of quantitative detection were lower than 0.06μg/kg,with the precision of none of them being more than 10%.【Conclusion】The method has a low quantitative detection line,simple step-by-step sample processing,high accuracy and good stability,and can meet the needs of trace detection in large quantities of beef samples.

关 键 词：莱克多巴胺 克伦特罗 沙丁胺醇 质谱 牛肉 

分 类 号：S859.84[农业科学—临床兽医学]