Acalypha australis L.extract inhibits B16 melanoma cell metastasis through PI3K/AKT signaling pathway  

作　　者：Zhi-Zhong Wang Tie-Shan Yi Yu-Yang He Qin Zhou Bo Chen 

机构地区：[1]College of Biology and Food Engineering,Huaihua University,Huaihua 418008,China [2]Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province,College of Biological and Food Engineering,Huaihua University,Huaihua 418008,China

出　　处：《Integrative Medicine Discovery》2024年第2期1-6,共6页

基　　金：This work was supported by the Hunan Education Department Project(NO.20A390);National Innovation and Entrepreneurship Training Program(S202010548007).

摘　　要：Background:Melanoma is a deadly skin tumor resulting from the malignant transformation of melanocytes.It is highly malignant and invasive,with the highest mortality rate among skin cancers.Acalypha australis L.(AAL),a plant with dual medicinal and culinary purposes,is commonly regarded as an edible wild vegetable in southern China.Additionally,AAL has a long history of medicinal use in China,often employed for its hemostatic,anti-diarrheal,and anti-inflammatory properties.Modern pharmacology has demonstrated that AAL possesses functions such as weight loss,antimicrobial activity,antiviral effects,and treatment for ulcerative colitis.However,there is currently no research available regarding its effectiveness and mechanisms of action on melanoma.Methods:In this investigation,we used methyl thiazolyl tetrazolium assay to detect cell viability,transwell assay to detect cell migration and invasion ability,and Western blot assay to detect relevant signaling pathways.Results:The present study reveals that 2 mg/mL AAL effectively suppresses the metastasis of B16 cells,while simultaneously triggering the expression of key apoptosis-related proteins,including Bcl-2,Bax,and cleaved caspased 3.Subsequent investigations demonstrate that AAL exerts this inhibitory effect via the PI3K/AKT signal transduction pathway,as evidenced by the observed deficits in Ras,AKT,p-AKT,and PI3K expression levels.Conclusion:These findings indicated that AAL could be a valuable therapeutic option for reducing the metastatic potential of B16 melanoma cells.

关 键 词：Acalypha australis L MELANOMA PI3K/AKT pathway 

分 类 号：R739.5[医药卫生—肿瘤]