硫酸软骨素低共熔溶剂法高效提取及其结构表征  

Efficient extraction and structural characterization of chondroitin sulfate by deep eutectic solvents

作　　者：王雯芳 王康宇 郭玉杰 张春晖[2] 李萍[1] 刘泓 WANG Wenfang;WANG Kangyu;GUO Yujie;ZHANG Chunhui;LI Ping;LIU Hong(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Comprehensive Key Laboratory of Agro-products Processing,Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]中国农业科学院农产品加工研究所,农业农村部农产品加工综合性重点实验室,北京100193

出　　处：《农业工程学报》2023年第23期276-284,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：新疆“两区”科技发展计划项目(2022 LQ01002);国家农业科技创新工程项目(CAAS-ASTIP-2021-IFST)。

摘　　要：硫酸软骨素(chondroitin sulfate,CS)是重要的功能性骨多糖,广泛分布于动物的软骨组织。为了提高动物软骨中CS提取率,该研究制备了12种天然低共熔溶剂(natural deep eutectic solvents,NADESs),基于CS提取率筛选了较佳NADESs组成、确定了CS较佳提取工艺条件,并考察了NADES的循环重用性。结果表明,NADES-3(甜菜碱/尿素,摩尔比1:2)的CS提取率较高,为16.49%。且在较佳提取条件(含水率40%、液料比30:1 mL/g、反应温度110℃、时间120 min)下,CS提取率可达17.33%,是传统水提法的2.88倍。NADESs的理化特性与CS提取率的相关性试验表明,提取率与黏度呈负相关,与pH值、极性呈正相关,与密度相关性不大,确定了含水率40%的NADES-3为该试验最佳提取CS的溶剂。回收试验表明,NADES-3重复使用5次后,CS提取率仍有15.89%,说明NADESs提取法能高效地从喉软骨中回收CS。研究结果为畜禽骨营养组分的高效利用提供新思路,也为后续运动健康类功能性骨源食品的开发提供参考。Chondroitin sulfate(CS)is widely distributed in animal cartilage tissues,such as thoracic cartilage,laryngeal bone,and nasal bone.The market demand for CS products is ever increasing with the global aging and aging of chronic diseases in recent years,due to the various functional characteristics,such as antioxidant,anti-inflammatory,and lipid-lowering.It is very necessary to promote the extraction of specific active substances.Natural deep eutectic solvents(NADESs)are often simply synthesized to design their structure for high extraction efficiency.The solvents can also be recycled and reused after use,in order to make them green and environmentally friendly.This study aims to improve the utilization rate of cartilage raw materials and reduce the burden of waste treatment.A natural deep eutectic solvent method was also proposed to extract the CS from the bovine laryngeal cartilage,taking betaine and choline chloride as hydrogen bond acceptors and six common natural substances as hydrogen bond donors.Twelve NADESs were prepared to combine them in pairs.Among them,NADES-3(betaine/urea,molar ratio 1:2)had the highest extraction rate of CS,which was 16.49%.The reason was that the weak hydrogen bonds were formed in the solvent,due to the small molecular weight of urea,and then the betaine counteracted the denaturation of protein by urea,indicating the synergistic effect.The extracted CS was characterized by monosaccharide analysis,infrared spectroscopy,and molecular weight analysis,in order to confirm the extracted CS with an excellent structure.Subsequently,the effects of four single factors on CS extraction were studied,including liquid-solid ratio(10:1,20:1,30:1,40:1,and 50:1 mL/g),moisture content(5%,20%,40%,60%,and 80%),temperature(90,100,110,120,and 130℃),and time(60,90,120,150,and 180 min).The optimal extraction conditions were:liquid-solid ratio of 30:1 mL/g,moisture content of 40%,extraction temperature of 110℃,and extraction time of 120 minutes.The CS extraction rate reached 17.33%,which was 2.88 times h

关 键 词：提取 天然低共熔溶剂 硫酸软骨素 溶剂性质 喉软骨 回收利用性 

分 类 号：TS209[轻工技术与工程—食品科学]