Transcriptomic and genomic identification of spliceosomal genes from Euglena gracilis  

作　　者：Pingwei Gao Yujie Zhong Chengfu Sun 

机构地区：[1]Scientific Research Center,Chengdu Medical College,Chengdu 610500,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第11期1740-1748,共9页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China (No.31872717);the Disciplinary Construction Innovation Team Foundation of Chengdu Medical College (No.CMC-XK-2102).

摘　　要：Diverse splicing types in nuclear and chloroplast genes of protist Euglena gracilis have been recognized for dec-ades.However,the splicing machinery responsible for processing nuclear precursor messenger RNA introns,including trans-splicing of the 5′terminal outron and spliced leader(SL)RNA,remains elusive.Here,we identify 166 spliceosomal protein genes and two snRNA genes from E.gracilis by performing bioinformatics analysis from a combination of next-generation and full-length transcriptomic RNA sequencing(RNAseq)data as well as draft genomic data.With the spliceosomal proteins we identified in hand,the insensitivity of E.gracilis to some splicing modulators is revealed at the sequence level.The prevalence of SL RNA-mediated trans-splicing is estimated to be more than 70%from our full-length RNAseq data.Finally,the splicing proteomes between E.gracilis and its three evolutionary cousins within the same Excavata group are compared.In conclusion,our study characterizes the spliceosomal components in E.gracilis and provides the molecular basis for further exploration of underlying splicing mechanisms.

关 键 词：txcavata SPLICEOSOME INTRON TRANSCRIPTOME TRANS-SPLICING SF3B 

分 类 号：Q78[生物学—分子生物学]