平阴玫瑰花色苷的鉴定及对LPS/AGEs/4-HNE诱导炎症的抑制  

Identification of Pingyin Rose Anthocyanins and Inhibition of LPS/AGEs/4-HNEInduced Inflammation

作　　者：吕雯雯 吴华[2] 肖俊松[1] 曹雁平[1] 雷胜明 LüWenwen;Wu Hua;Xiao Junsong;Cao Yanping;Lei Shengming(Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Engineering and Technology Research Center of Food Additives,College of Food and Health,Beijing Technology and Business University,Beijing 100048;College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048;Jinan Zijin Rose Co.,Ltd.,Jinan 250000)

机构地区：[1]北京工商大学食品与健康学院、北京市食品添加剂工程技术研究中心食品添加剂与配料北京高校工程研究中心,北京100048 [2]北京工商大学化学与材料工程学院,北京100048 [3]济南紫金玫瑰股份有限公司,济南250000

出　　处：《中国食品学报》2023年第12期37-50,共14页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市自然科学基金项目(6212002);北京市教委一般项目(KM202010011010)。

摘　　要：平阴玫瑰是我国山东特有的玫瑰花资源,于2010年被批准为新资源食品。本文提取并鉴定平阴玫瑰花中的花色苷,以脂多糖(LPS)、晚期糖基化终末产物(AGEs)、4-羟基壬烯醛(4-HNE)分别诱导RAW264.7巨噬细胞构建炎症模型,研究其对活性氧(ROS)、一氧化氮(NO)生成及部分炎症因子表达的影响。结果表明:平阴玫瑰花色苷主要含有2种苷元,分别为矢车菊素和芍药色素;共鉴定出6种主要花色苷,分别为3种矢车菊素-二己糖苷、2种芍药素-二己糖苷及芍药素-芸香糖-己糖苷。花色苷能显著抑制LPS、AGEs和4-HNE诱导的RAW264.7细胞中ROS和NO的产生,显著抑制促炎因子IL-1β、IL-6、TNF-α、iNOS的表达,表现出较好的抑制炎症反应的效果。Pingyin rose was approved as a new resource food in 2010,which is a unique rose resource food in Shandong province of China.Anthocyanins were extracted and preliminarily identified from Pingyin rose.RAW264.7 macrophages were induced by lipopolysaccharide(LPS),advanced glycation end products(AGEs)and 4-hydroxynonenal(4-HNE)to construct inflammatory model.The effects of anthocyanins on the production of reactive oxygen species(ROS)and nitric oxide(NO)and the expression of some inflammatory factors in RAW264.7 cells were studied.Results showed that rose anthocyanins mainly contained two aglycones,namely cyanidin and peonidin.A total of six anthocyanins were identified,including three cyanidin-dihexoses,two peonidin dihexoses and peonidin-rutinose-hexose.Anthocyanins could significantly inhibit the production of ROS and NO and the expression of IL-1β,IL-6,TNF-α,iNOS in RAW264.7 cells,showing a good inhibitory effect on inflammation.

关 键 词：平阴玫瑰 花色苷 鉴定 炎症 抑制 RAW264.7细胞 

分 类 号：TS201.4[轻工技术与工程—食品科学]