米糠酚类物质对高脂诱导的小鼠糖代谢异常及氧化应激的改善作用  

Effect of Rice Bran Phenolic Extracts on Abnormal Glucose Metabolism and Oxidative Stress Induced by High-fat Diet in Mice

作　　者：马勤 童鑫[1] 张名位[1] 董丽红[1] 黄菲[1] 赵东 池建伟[1] 贾栩超 张瑞芬[1] Ma Qin;Tong Xin;Zhang Mingwei;Dong Lihong;Huang Fei;Zhao Dong;Chi Jianwei;Jia Xuchao;Zhang Ruifen(Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory Foods,Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory Processing,Guangzhou 510640)

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所、农业农村部功能食品重点实验室、广东省农产品加工重点实验室,广州510640

出　　处：《中国食品学报》2024年第1期119-125,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：广东特支计划本土创新团队项目(2019BT02N112);广东省农业科学院人才引进项目(R2019YJ-YB1003);广东省基础与应用基础研究基金项目(2019A1515110633)。

摘　　要：目的:研究不同剂量的米糠酚类物质(RBPE)对高脂诱导的小鼠糖代谢及氧化应激水平的影响,为揭示米糠改善糖代谢作用提供理论依据。方法:选用6周龄的C57BL/6J小鼠适应性饲养1周后,随机分为4组,包括正常组、高脂组、RBPE低剂量组(200 mg/kg,HF+LP组)和高剂量组(400 mg/kg,HF+HP组),实验共16周,测定脏器指数、脂肪细胞形态、糖代谢指标和氧化应激相关指标。结果:RBPE可以剂量依赖地降低小鼠肝脏、附睾脂肪和肾周脂肪的脏体比,降低高脂小鼠空腹血糖水平,改善高脂引起的糖耐量受损(P<0.05):与高脂组相比,HF+LP、HF+HP组小鼠肝脏的脏体比分别降低15.75%和39.61%,附睾脂肪的脏体比分别降低23.03%和39.83%,肾周脂肪的脏体比分别降低43.68%和56.83%,空腹血糖水平分别降低14.06%和20.21%,糖耐量曲线下面积分别降低19.06%和23.81%。进一步研究显示,低、高剂量的RBPE可显著降低高脂小鼠血清和肝脏组织MDA的含量并提高SOD活力,与高脂组相比,HF+HP组小鼠血清和肝脏MDA含量分别降低了48.93%和38.68%,SOD活力分别提高了80.44%和67.05%;并提高了高脂诱导小鼠肝脏过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-px)等抗氧化酶的活力及抗氧化物谷胱甘肽(GSH)的含量(P<0.05),与高脂组相比,HF+HP组小鼠肝脏CAT、GSH-px活力及GSH含量分别提高了48.49%,31.67%,123.90%。结论:米糠酚类物质可以改善高脂诱导的小鼠糖代谢紊乱,其机制可能与改善机体氧化应激反应有关。Objective:This study was conducted to investigate the effects of rice bran phenolic extracts(RBPE)supplementation on glucose metabolism and oxidative stress in high-fat diet induced mice,which could provide theoretical basis for revealing the effect of rice bran on glucose metabolism.Methods:After 1 week of acclimatization,mice were randomly divided into 4 groups:normal control group,high-fat diet group,low-dose RBPE group(200 mg/kg,HF+LP group)and high-dose RBPE group(400 mg/kg,HF+HP group).The experiment lasted for 16 weeks.The organ-body ratio,epididymal fat morphology,fasting serum glucose level,oral glucose tolerance and the levels of oxidative stress were measured.Results:RBPE dose-dependently decreased the organ-body ratio,fasting blood glucose and ameliorated the impaired glucose tolerance induced by high-fat diet.Compared with the high-fat diet group,organ-body ratio of the liver of HF+LP and HF+HP groups were decreased by 15.75%and 39.61%,respectively,the organ-body ratio of epididymal adipose tissue decreased by 23.03%and 39.83%,respectively,the organ-body ratio of renal peritoneal adipose tissue decreased by 43.68%and 56.83%,respectively,fasting blood glucose levels decreased by 14.06%and 20.21%,and the area under the glucose tolerance curve decreased by 19.06%and 23.81%,respectively.Further studies showed that low and high doses of RBPE can significantly reduce serum and liver tissue MDA content and increase SOD activity in high-fat diet induced mice.Compared with the high-fat diet group mice,serum and liver MDA content in HF+HP group mice were decreased by 48.93%and 38.68%,respectively,SOD activity increased by 80.44%and 67.05%,respectively;RBPE treatment could also increase antioxidant enzyme activities such as catalase(CAT)and glutathione peroxidase(GSH-px)and the content of antioxidant glutathione(GSH)in liver tissue of high-fat diet induced mice(P<0.05).Compared with the high-fat diet group mice,CAT,GSH-px activity and GSH content in HF+HP group mice were increased by 48.49%,31.67%and 123.90%,re

关 键 词：米糠酚类物质 高脂 糖代谢 氧化应激 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]