2021年河南省基层疾控机构单核细胞增生李斯特菌实验室间比对结果分析  

Analysis of inter-laboratory comparison results of Listeria monocytogenes detection among the disease control institutions at the grass roots in Henan province in 2021

作　　者：李艳芬[1] 张濛[1] 吴玲玲[1] 崔莹[1] 张广伟[1] 邱正勇[1] LI Yanfen;ZHANG Meng;WU Lingling;CUI Ying;ZHANG Guangwei;QIU Zhengyong(Henan Center for Disease Control and Prevention/Henan Provincial Key Laboratory of Foodborne Disease Pathogen Traceability Medicine,Zhengzhou,Henan 450016,China)

机构地区：[1]河南省疾病预防控制中心/河南省食源性疾病病原学溯源医学重点实验室,河南郑州450016

出　　处：《现代疾病预防控制》2024年第1期56-58,75,共4页MODERN DISEASE CONTROL AND PREVENTION

基　　金：河南省医学科技攻关省部共建项目(SBGJ202103030);河南省医学科技攻关联合共建项目(LHGJ20190678)。

摘　　要：目的 通过河南省疾控机构实验室间比对,科学评估基层疾控机构对食品中单核细胞增生李斯特菌检测技术水平,确保检测结果准确可靠。方法 制备3类混合菌A、B、C的质控样品,于2021年5月发放给全省36家基层疾控机构,依据GB 4789.30—2016进行定性检测和鉴定,根据检测结论和操作步骤评定各参与实验室的测试结果。结果 样品B和C合格率均达到100.0%,A样品的合格率最低,为83.3%(30/36)。18家省辖市、示范区级实验室合格率为100.0%,18家市县(区)级实验室合格率为66.7%(12/18)。不同行政级别疾控机构实验室间比对合格率存在统计学差异(χ^(2)=5.250,P<0.05)。36家实验室全部采用溶血试验完成单核细胞增生李斯特菌最终确认鉴定。结论 应着重加强市县(区)级疾控机构关于食品中单核细胞增生李斯特菌的检测水平。Objective To scientifically evaluate the technical level of detection of Listeria monocytogenes in food by primary disease control institutions and ensure the accuracy and reliability of the test results through the inter-laboratory comparison among disease control institutions at the grass roots in Henan province. Methods The quality control samples of three kinds of mixed bacteria A, B and C were prepared and distributed to 36 disease control institutions at the grass roots in the province in May 2021. Qualitative detection and identification were carried out according to GB 4789.30-2016, and the test results of each participating laboratory were evaluated according to the test conclusions and operation steps. Results The qualified rate for samples B and C reached 100.0%, and the qualified rate for sample A was the lowest, which was 83.3%(30/36). The qualified rate in the laboratories of the 18 prefecture-level cities and demonstration areas was 100.0%, and the qualified rate in the laboratories of 18 cities and counties(districts) was 66.7%(12/18). There was a statistical difference in the qualified rate of inter-laboratory comparison among different administrative levels of disease control institutions( χ^(2)=5.250, P<0.05). All 36laboratories completed the final confirmation and identification of Listeria monocytogenes by hemolysis test. Conclusions It should be focused on strengthening the detection level of Listeria monocytogenes in food in the disease control institutions at city/county(district) level.

关 键 词：河南省 基层疾控 食品 单核细胞增生李斯特菌 实验室间比对 

分 类 号：R155.5[医药卫生—营养与食品卫生学]