大蒜素有效成分DATS对粪肠球菌抑制作用的体外研究  

Inhibitory effects of allicin effective component DATS on Enterococcus faecalis bofilm:An in vitro Study

作　　者：王立媛[1] 申思敏 白锡静 WANG Liyuan;SHEN Simin;BAI Xijing(Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction,Tianjin Stomatological Hospital,School of Medicine,Nankai University,China,300041)

机构地区：[1]天津市口腔功能重建重点实验室,天津市口腔医院·南开大学口腔医院,300041

出　　处：《实用口腔医学杂志》2024年第1期104-108,共5页Journal of Practical Stomatology

基　　金：天津市卫生健康科技中西医结合项目(编号:TJWJ2021150);天津市医学重点学科(专科)建设项目(编号:TJYXZDXK-078D)。

摘　　要：目的:观察大蒜素有效成分二烯丙基化三硫(DATS)对感染根管粪肠球菌生物膜的作用。方法:选取60颗健康单根管恒牙,45颗建立粪肠球菌感染根管模型。分为4组(n=15):阴性对照组(未感染组)、DATS组、氢氧化钙组和阳性对照(无抗菌处理)组。二倍稀释法测定DATS对粪肠球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。分别于实验第1天、第3天和第7天,取根管内层牙本质粉末,置于2 mL BHI中培养72 h,电子比浊仪读取肉汤上层液体浊度。使用SPSS 26.0统计软件进行数据分析。结果:DATS对粪肠球菌的MIC和MBC(μg/mL)分别为2 560和5 120。第1天,DATS组、氢氧化钙组、阳性对照组与阴性对照组浑浊度,DATS组小于氢氧化钙组(P<0.05);第3天时,DATS组与阴性对照组浑浊度无统计学差别(P=0.454),已基本杀灭细菌;第7天时,氢氧化钙糊剂组与阴性对照组有统计学差别(P<0.05),不能完全杀灭细菌。各时间点阳性对照组浑浊度最高。结论:DATS能够有效杀灭或抑制感染根管中的粪肠球菌。Objective:To investigate the antibacterial efficacy of allicin effective component,dially trisulfide(DATS),on the biofilms of Enterococcus faecalis(E.faecalis)in vitro.Methods:60 healthy single root canal permanent teeth were selected.After cleaning and autoclaving,15 teeth without infection were used in the negative control group,45 teeth were contaminated by E.faecalis and incubated for 28 days as the infection models and devided into 3 groups(n=15)as below:DATS group,Calcium hydroxide group and positive(without antibacteria treatment)control group.The minial inhibition concentration(MIC)and minial bactericidal concentrition(MBC)of DATS on E.feacalis were studied with doubling diluting method.At the day 1,2 and 7 of the exprement,the 4 mg of root canal inner dentin powder,5 specimens of each group were sespectively transfered into sterile bottle containing 2 mL BHI broth and cultured at 37℃5% CO_(2) for 72 h,the turbitity of the upper layer of liquid of the culture was measured.Results:MIC and MBC(μg/mL)of DATS to E.feacalis was 2560 and 5120 repsectively.At the first day,turbidity in the DATS group was the lowest(P<0.05).At the third day,turbidity showed no difference between DATS group and negative control group(P=0.454).At the 7th day,between calcium hydroxide and the negative control group,P<0.05.Conclusion:DATS may suppress E.faecalis in the biofilm in vitro.

关 键 词：大蒜素 二烯丙基化三硫 粪肠球菌 氢氧化钙 根管消毒 

分 类 号：R781.3[医药卫生—口腔医学] R780.2[医药卫生—临床医学]