基于出芽短梗霉发酵法制备β-聚苹果酸  

Preparation ofβ-PMLA Based on the Fermentation of Aureobasidium Pullulans

作　　者：谭昕 康建平 李佳逸 何雨静 王燕来 何勇[1] TAN Xin;KANG Jianping;LI Jiayi;HE Yujing;WANG Yanlai;HE Yong(Sichuan Textile Scentific Research Institute Co.,Ltd.,Chengdu 610083,China;High Tech Organic Fibers Key Laboratory of Sichuan Province,Chengdu 610083,China)

机构地区：[1]四川省纺织科学研究院有限公司,成都610083 [2]高技术有机纤维四川省重点实验室,成都610083

出　　处：《纺织科技进展》2024年第1期15-18,25,共5页Progress in Textile Science & Technology

基　　金：高技术有机纤维四川省重点实验室开放基金资助项目(PLN2022-17)。

摘　　要：通过改变出芽短梗霉Aureobasidium pullulans的培养与发酵的工艺条件,实现β-聚苹果酸的生成量和分子量的提高。试验表明,最佳培养条件和培养组分为:100 g/L葡萄糖,35 g/L蛋白胨,2 g/L丁二酸铵,0.1 g/L KH2PO4,0.3 g/L MgSO4·7H2O,0.5 g/L KCl, 0.05 g/L MgSO4,20 g/L CaCO3,0.5 g/L亮氨酸,pH值4.0,发酵温度25℃,培养时间6 d。在该条件下进行发酵培养,β-PMLA的产量提高到24.3 g/L,分子量提高到8 948 Da。The process conditions of culture and fermentation of Aureobasidium pullulans were varied to achieve an increase in the production and molecular weight of polyβ-malic acid.The optimal culture conditions and culture components were shown to be:glucose 100 g/L,peptone 35 g/L,ammonium succinate 2 g/L,KH_(2)PO_(4)0.1 g/L,MgSO_(4)·7H_(2)O 0.3 g/L,KCl 0.5 g/L,MgSO_(4)0.05 g/L,CaCO_(3)20 g/L,leucine 0.5 g/L,pH value 4.0,fermentation temperature 25℃ for 6 d.Under these conditions,the yield ofβ-PMLA increased to 24.3 g/L and the molecular weight increased to 8948 Da.

关 键 词：出芽短梗霉 β-聚苹果酸 发酵 工艺优化 

分 类 号：TQ921[轻工技术与工程—发酵工程]