谷氨酰胺、姜黄素组方对酒精性胃粘膜损伤的保护作用  

Protective Effect of a Combined Glutamine and Curcumin Formulation on Alcoholic Gastric Mucosal Damage

作　　者：陆海霞 徐莹 潘美辰 唐天培 赵建云[1] 葛磊 LU Haixia;XU Ying;PAN Meichen;TANG Tianpei;ZHAO Jianyun;GE Lei(Hangzhou Wahaha Group Company Limited,Key Laboratory of Food and Biological Engineering of Zhejiang Province,Hangzhou 310018,China)

机构地区：[1]杭州娃哈哈集团有限公司,浙江省食品生物工程重点实验室,浙江杭州310018

出　　处：《食品工业科技》2024年第4期299-304,共6页Science and Technology of Food Industry

摘　　要：目的:旨在探讨谷氨酰胺、姜黄素组方对乙醇致大鼠胃粘膜损伤的保护作用及机制。方法:将50只SPF级健康SD雄性大鼠随机分为空白组、模型对照组、西米替丁组、高剂量组、低剂量组5组。连续30 d口服灌胃给药后,除空白组外,其余各组均在无水乙醇造模后处死。通过H&E染色观察胃粘膜组织病理学变化情况,采用试剂盒测定血清丙二醛(malondialdehyde,MDA)、一氧化氮(NO)含量和谷胱甘肽过氧化物酶(Glutathioneperoxidase,GSH-Px)活性,并测定组织中前列腺素E_(2)(PGE_(2))含量水平和血红素加氧酶-1(heme oxygenase-1,HO-1)、NADPH醌氧化还原酶(NADPH Quinone Oxidoreductase 1,NQO1)、抗氧化相关基因核因子-E_(2)相关因子2(Nuclear factor-E_(2)related factor2,Nrf2)、丝氨酸蛋白激酶-3β(Glycogen synthase kinase-3β,GSK-3β)的表达情况。结果:西米替丁组、高剂量给药组的胃粘膜出血等损伤情况较模型组(P<0.05)均有所缓解,高剂量给药组的效果更明显。此外,模型组MDA含量和GSH-PX活性明显增加,NO和PGE_(2)含量明显下降(P<0.05),抗氧化相关基因HO-1、NQO1和Nrf2表达受到明显抑制,GSK-3β表达明显增加。与模型组相比,西咪替丁组和高剂量给药组MDA含量和GSH-Px活性显著下降,NO、PGE_(2)含量显著上升(P<0.05),抗氧化相关基因HO-1、NQO1、Nrf2得到显著恢复(P<0.05),GSK-3β被抑制(P<0.05)。结论:组方对乙醇引起的急性胃黏膜损伤有抑制作用,其作用机制推测与Keap1-Nrf2-ARE氧化应激通路有关。Objective:This study aimed to investigate the protective effect and underlying mechanism of a combined glutamine and curcumin formulation on ethanol-induced gastric mucosal damage in rats.Method:A total of fifty SPF-grade healthy SD male rats were randomly partitioned into five groups:A normal group,a model control group,a cimetidine group,a high-dose treatment group,and a low-dose treatment group.After a period of 30 days marked by oral gavage administration,all groups,with the exception of the normal group,were euthanized post anhydrous ethanol-induced modeling.The histopathological alterations in the gastric mucosa were observed via hematoxylin&eosin(H&E)staining.Furthermore,serum levels of malondialdehyde(MDA),nitric oxide(NO),and glutathione peroxidase(GSH-PX)were ascertained using a specific reagent kit.Concurrently,the concentration of prostaglandin E_(2)(PGE_(2))within the tissue and the expression levels of heme oxygenase-1(HO-1),NADPH quinone oxidoreductase(NQO1),the antioxidant-related nuclear factor-E_(2)-related factor 2(Nrf2)gene,and glycogen synthase kinase-3β(GSK-3β)were evaluated.Results:In the cimetidine and high-dose treatment groups,the incidence of gastric mucosal bleeding and other forms of injury were noticeably mitigated(P<0.05)compared to the model control group,with the high-dose treatment group demonstrating a more pronounced effect.Moreover,the model control group exhibited a significant elevation in MDA content and GSH-PX activity and a concurrent decline in NO and PGE_(2) levels(P<0.05).The expression of antioxidant-related genes,namely,HO-1,NQO1,and Nrf2,was significantly suppressed(P<0.05),whereas GSK-3βexpression was markedly increased.In contrast,in comparison to the model control group,the cimetidine and high-dose treatment groups manifested a significant reduction in MDA content and GSH-PX activity,while NO and PGE_(2) levels notably increased(P<0.05).The expression of the antioxidant-related genes HO-1,NQO1,and Nrf2 was significantly returned to normal(P<0.05),and GSK-3βex

关 键 词：谷氨酰胺 姜黄素 胃粘膜损伤 氧化应激 

分 类 号：TS201.4[轻工技术与工程—食品科学]