采用单孢杂交技术判定金耳极性  

Determination of Polarity of Naematelia aurantialba by Single Spore Hybridization

作　　者：曹瑶 沈真辉 杨林雷 李梦杰 罗祥英 杨晓君 陆青青 李荣春 CAO Yao;SHEN Zhenhui;YANG Linlei;LI Mengjie;LUO Xiangying;YANG Xiaojun;LU Qingqing;LI Rongchun(Yunnan Junshijie Biotechnology Ltd.,Yunnan(Rare Edible Fungi)Enterprise Technology Center,Kunming(Edible Fungi)Enterprise Technology Center,Kunming 650200,Yunnan,China;Institute of Edible Fungi,Yunnan Agricultural University,Kunming 650201,Yunnan,China)

机构地区：[1]云南菌视界生物科技有限公司,云南省级(珍稀食用菌)企业工程技术中心,昆明市级(食用菌)企业工程技术中心,云南昆明650200 [2]云南农业大学食用菌研究所,云南昆明650201

出　　处：《食用菌学报》2024年第1期38-44,共7页Acta Edulis Fungi

基　　金：国家自然科学基金-云南联合项目(U1802231)。

摘　　要：收集成熟金耳(Naematelia aurantialba)担孢子,单孢分离,经核染色验证共得到50个酵母状单孢分离物;栽培实验结果表明酵母状单孢分离物与毛韧革菌(Stereum hirsutum)共培养未出菇。第一轮交配实验结果表明可亲和率为24.5%,再经过三轮交配实验确定金耳单孢分离物具有4种交配型A_(1)B_(1)(13个)、A_(1)B_(2)(13个)、A_(2)B_(1)(12个)、A_(2)B_(2)(12个),初步表明金耳属于四极性异宗结合真菌。从4种交配型单孢分离物中分别选取5个进行两两交配及栽培实验,结果表明当2个单孢分离物的A、B交配型均不同时交配形成双核体,其与毛韧革菌(S.hirsutum)共培养方可生长发育形成金耳子实体。Basidiospores were collected from mature fruiting bodies of Naematelia aurantialba,and eventually a total of 50 yeast-like single spore isolates were obtained through single spore isolation and nuclear staining.Cocultivation of such a yeast-like single spore isolate with Stereum hirsutum failed to produce fruiting bodies.The first round of mating test showed that the compatibility was 24.5%.After another three rounds of mating tests,it was determined that there were four mating types,A_(1)B_(1)(13),A_(1)B_(2)(13),A_(2)B_(1)(12),and A_(2)B_(2)(12),suggesting that N.aurantialba belongs to quadrupole heterothallism fungi.Then five single spore isolates were randomly selected from each mating type for pairwise mating and cultivation experiments.The results showed that mature fruiting bodies of N.aurantialba were developed when co-culturing single spore isolates that can form dikaryons with the mating type A_(1)A_(2)B_(1)B_(2) together with S.hirsutum.

关 键 词：金耳 毛韧革菌 单孢分离物 交配型 

分 类 号：S646[农业科学—蔬菜学]