栝楼根腐病镰孢菌拮抗菌SJ1623发酵代谢物的抑制活性  

Inhibitory Activity of Fermentation Metabolites of Antagonistic Bacteria SJ1623 Against Trichosanthes kirilowii Fusarium Root Rot

作　　者：余利[1] 孟祥涛 陈甦 潘怀松 段海明[1] YU Li;MENG Xiang-tao;CHEN Su(Anhui Science and Technology University,Fengyang,Anhui 233100)

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《安徽农业科学》2024年第3期119-121,共3页Journal of Anhui Agricultural Sciences

基　　金：凤阳县科技计划项目(NY2022-05);作物抗逆育种与减灾国家地方联合工程实验室开放基金(NELCOF20190102);安徽省高校自然科学研究项目(KJ2017A510,KJ2019A0814);安徽省重点研究与开发计划项目(202104a06020001)。

摘　　要：检测了6株拮抗菌发酵上清液对栝楼根腐病菌的抑制活性,采用菌丝生长速率法测定优势拮抗菌不同接种量和发酵时间对发酵上清液抑菌活性的影响,测定了不同稀释倍数的发酵上清液和脂肽粗提物对栝楼根腐病菌的抑制活性。结果表明,对栝楼根腐病菌抑制活性最高的拮抗菌为SJ1623,种子液培养时间为10 h,0.75%(体积比)的接种量效果较好;发酵上清液稀释10倍对病菌的抑制率为87.6%,脂肽粗提物50倍稀释液对病菌的抑制率为83.1%。The inhibitory activity of the fermentation supernatant of 6 antagonistic bacteria against Trichosanthes kirilowii Fusarium root rot was detected.The effects of different inoculation amount and fermentation time of the dominant antagonistic bacteria on the antifungal activity of fermentation supernatant were determined by the mycelial growth rate method.The inhibitory activity of fermentation supernatant and crude lipopeptide extract against Fusarium sp.was determined.The results showed that the antagonistic bacteria SJ1623 had the highest inhibitory activity.The seed liquid culture time was 10 h,and the inoculation amount of 0.75%(volume ratio)was better.The inhibition rate of the fermentation supernatant diluted 10 times was 87.6%.The inhibition rate of 50 times dilution of crude lipopeptide extract was 83.1%.

关 键 词：栝楼根腐病 拮抗菌 发酵液 抑制活性 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]