基于响应面法优化沙棘普洱茶粉水提工艺及体外抗氧化能力评价研究  

Optimization of water extraction process of Seabuckthorn Pu′er tea powder and evaluation of its antioxidant capacity in vitro based on response surface methodology

作　　者：张靖鹏 翟宇晴 张得钧 ZHANG Jing-peng;ZHAI Yu-qing;ZHANG De-jun(College of Eco-environmental Engineering,Qinghai University,Xinin 810016,China)

机构地区：[1]青海大学生态环境工程学院,青海西宁810016

出　　处：《云南民族大学学报（自然科学版）》2024年第1期31-40,共10页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(2020ZJ922).

摘　　要：以沙棘普洱茶粉水提液中可溶性固形物为响应值,基于单因素及Box-Benhnken响应面法优化实验研究不同料液比、时间、温度、pH的影响.对不同原料配比下的沙棘普洱茶粉进行有效成分检测及体外抗氧化实验,分析、评价其体外抗氧化能力.得到沙棘普洱茶粉(沙棘叶∶普洱茶末=4∶1)的最优水提工艺条件为料液比(原料∶水)为1∶8(g/mL)、提取温度为79℃、提取时间为3 h、pH为8.3.沙棘普洱茶粉的活性成分含量较单一茶粉有所提高,对DPPH(2,2,-diphenyl-1-picrylhydrazyl)、ABTS(2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)、CuPRAC(Cupric ReducingAntioxidant Power)及FRAP(Ferric Reducing Antioxidant Power)均具有较强的清除能力,制备的沙棘普洱茶粉均具有较好的体外抗氧化作用,为沙棘、普洱茶资源利用及功能性茶粉的开发提供一定参考.Seabuckthorn Pu′er tea powder:(1)Taking the soluble solids in the water extract as the response value,based on the single factor and Box-Benhnken response surface method,the effects of different solid-liquid ratio,time,temperature and pH on the water extraction effect of Seabuckthorn Pu′er tea powder were studied.(2)In order to analyze and evaluate the antioxidant capacity in vitro,the effective components of Seabuckthorn Pu′er tea powder with different raw material ratios were tested and the antioxidant activity in vitro was tested.Results:(1)The optimum water extraction conditions for obtaining Seabuckthorn Pu′er tea powder(Seabuckthorn leaf:Pu′er tea powder=4∶1)were as follows:the ratio of material to liquid(raw material:water)was 1∶8(g/mL),the extraction temperature was 79℃,the extraction time was 3 h,and the pH was 8.3.(2)The content of active components of Seabuckthorn Pu′er tea powder is higher than that of single tea powder,and it has strong scavenging ability to DPPH(2,2,-diphenyl-1-piperylhydrazyl),ABTS(2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid),CuPRAC(Cupric Reducing Antioxidant Power)and FRAP(Ferric Reducing Antioxidant Power),which can provide some reference for the utilization of Seabuckthorn and Pu′er resources and the development of functional tea powder.

关 键 词：沙棘茶粉 普洱茶粉 水提工艺 响应面法 体外抗氧化 

分 类 号：TS272.4[农业科学—茶叶生产加工]