腐烂阿克苏红富士苹果中毒素的检测方法与迁移规律的研究  

Decay in the Aksu Fuji Apple Types and Content Determination of the Toxin

作　　者：吴思雅 姜楠[2] 韦迪哲 庞学群[3] 朱璇 WU Siya;JIANG Nan;WEI Dizhe;PANG Xuequn;ZHU Xuan(Food and Biotechnology Branch,Xinjiang Polytechnic of Light Industry Urumchi Xinjiang,Urumqi,Xinjiang 831499,China;Agricultural Quality Standard and Testing Technology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642,China;College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi,Xinjiang 830052,China)

机构地区：[1]新疆轻工职业技术学院食品与生物技术分院,新疆乌鲁木齐831499 [2]北京市农林科学院农业质量标准与检测技术研究中心,北京100097 [3]华南农业大学生命科学学院,广东广州510642 [4]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《农产品加工》2024年第1期70-75,共6页Farm Products Processing

基　　金：国家科技支撑计划项目(2015BAD16B07);校级课题(XJQG2020522)。

摘　　要：为了明确腐烂苹果中的6种真菌毒素的含量及其迁移规律。采用超高效液相色谱-质谱技术(UPLC-MS/MS)结合超高效液相色谱(HPLC),通过对腐烂的阿克苏红富士苹果中交链孢酚-AOH(Alternariol)、交链孢酚单甲醚-AME(Alternariol monamethyl ether)、细交链格孢酮酸-TEA(Tenuazonic acid)、交链孢烯-ALT(Altenuene)、腾毒素-TEN(Tentoxin)、展青霉素-PAT(Patulin)6种毒素的产毒范围、产毒条件、检测方法及迁移规律等方面进行了相关研究。结果表明,交链孢毒素广泛存在于霉变的苹果中,且随着贮藏时间的延长毒素不断累积;扩展青霉产生展青霉素的最适温度为20~25℃,且随着培养时间的延长,产毒量呈现增加的单峰性变化趋势,同时高湿条件有利于展青霉素的产生。Fuji apple in the growth,harvesting,transportation,processing and storage,and a series of process,vulnerable to infection of pathogenic microorganisms and decay,apple postharvest decay caused by pathogens had cross-linking of spore(Alternaria alternata)and the expansion of Penicillium(Penicillium expansum);Chain spore mold toxin was a series of metabolites produced by chain,spore fungus:cross-linking cross-linking spore of phenol(Alternariol AOH),spore phenol monomethyl ether(Alternariol monamethyl,AME)cross-linking,fine lattice spore keto acid(Tenuazonic acid,TeA),cross-linking spore ene(Altenuene,ALT),teng toxin(Tentoxin,TEN);Extension Penicillium(Penicillium expansum l.)would produce a toxic metabolite,namely patulin(PAT).Rotten apple not only caused huge economic losses,but also in the accumulation of a large number of fungus toxin in apple.Fungal toxins through the food chain transfer bring great threat to the health of people or animals.In this paper,the apple in the common type,enterotoxigenic strains of fungus toxin,infection way and toxin-producing conditions,toxic effects,such as detection method for the related research,provided a reference for apple mycotoxins in control.

关 键 词：真菌毒素 苹果 含量 检测 链格孢霉-AT 展青霉素-PAT 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]