珍稀濒危物种无距淫羊藿叶绿体基因组结构特征与系统发育分析  

Structural Characterization and Phylogenetic Analysis of the Chloroplast Genome of the Rare and Endangered Species of Epimedium ecalcaratum

作　　者：叶娟 魏升华 何顺志 罗琴 丁宁 王新村 严福林 YE Juan;WEI Shenghua;HE Shunzhi;LUO Qin;DING Ning;WANG Xincun;YAN Fulin(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Sinopharm Group Tongjitang(Guizhou)Pharmaceutical Co.,LTD,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵阳550025 [2]国药集团同济堂(贵州)制药有限公司,贵阳550025

出　　处：《种子》2023年第11期30-37,48,共9页Seed

基　　金：贵州省科技计划项目(黔科中引地[2022]4016);贵州省中药材现代产业技术体系建设(GZCYTX2019-2024)。

摘　　要：无距淫羊藿(Epimedium ecalcaratum G.Y.Zhong)为小檗科淫羊藿属形态特殊的类群之一,其分布狭窄,被IUCN列为濒危物种。本研究对无距淫羊藿叶绿体基因组结构及系统发育进行分析,以期为其遗传保护提供理论依据。结果表明,无距淫羊藿叶绿体基因组长度为164962 bp,包括大单拷贝区(LSC)80562 bp,小单拷贝区(SSC)17040 bp以及两个反向重复序列(IRa和IRb)各33680 bp,总GC含量为38.7%;无距淫羊藿叶绿体基因组共编码139个基因,包括94个蛋白编码基因,37个转运RNA基因,8个核糖体RNA基因。序列鉴定共发现3种类型的SSR,其中,单核苷酸68个、二核苷酸2个,复合核苷酸4个;21个基因包含1个或2个内含子;94个蛋白编码基因编码51997个密码子;系统发育分析结果支持淫羊藿属为单系,无距淫羊藿与分布较广的粗毛淫羊藿亲缘关系较近。Epimediumecalcaratum G.Y.Zhong is one of the special morphological groups of Berberaceae Epimedium.Its narrow distribution is listed as endangered species by IUCN.In this study,the chloroplast genome structure and phylogeny were analyzed in order to provide theoretical basis for its genetic protection.The results showed that the length of chloroplast genome was 164962 bp,including large single copy(LSC)80562 bp,small single copy region(SSC)17040 bp and two reverse repeats(IRa and IRb)33680 bp,with a total GC content of 38.7%.A total of 139 genes were encoded in the chloroplast genome,including 94 protein coding genes,37 transfer RNA genes,and 8 ribosomal RNA genes.Three types of SSR were identified,including 68 mononucleotides,2 dinucleotides and 4 complex nucleotides.21 genes contained one or two introns;94 protein-coding genes encoded 51997 codons;Phylogenetic analysis showed that Epimedium was monophyletic,and it was closely related to the widely distributed epimedium.

关 键 词：无距淫羊藿 叶绿体基因组 SSR 密码子偏好性 

分 类 号：S567.239[农业科学—中草药栽培]