红枣环磷腺苷的提取工艺优化及应用研究  

Study on extraction process optimization and application of cyclic adenosine monophosphate from jujube

作　　者：龚频 龙辉 王小娟 杨文娟 常相娜 陈福欣[2] GONG Pin;LONG Hui;WANG Xiaojuan;YANG Wenjuan;CHANG Xiangna;CHEN Fuxin(School of Food and Biological Engineering,Shaanxi University of Science and Technology,Xi′an 710021,China;School of Chemistry and Chemical Engineering,Xi′an University of Science and Technology,Xi′an 710054,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]西安科技大学化学与化工学院,陕西西安710054

出　　处：《食品与发酵工业》2024年第2期184-190,共7页Food and Fermentation Industries

基　　金：西安市未央区科研创新基金(202036);榆林市科技计划项目(2019-197);陕西省科技厅重点产业创新链(群)-农业领域(2021ZDLNY04-01);陕西省科技厅一般项目(2019GY-138,2020GY-236,2021NY-161);西安市科技项目(21NYYF0022)。

摘　　要：该实验对红枣中环磷腺苷的提取工艺和应用进行了研究,通过单因素试验分别考查了料液比、提取时间、乙醇体积分数3个单因素对于环磷腺苷得率的影响,确定了在环磷腺苷得率最高的情况下的各因素的最适条件,并利用得到的提取物制备了乳霜。利用Design-Expert V8.0.6软件进行中心组合设计法设计响应面的实验组,通过实验得到的数据建立模型,得到环磷腺苷的最优提取工艺为:料液比1∶25.42(g∶mL)、提取时间63.51 min、乙醇体积分数61.63%,并对乳霜稳定性进行检测。在最佳提取工艺下,环磷腺苷得率为189.07μg/g,并利用提取物制备得到一款具有良好稳定性的乳霜。This paper aimed to improve the utilization value of jujube and develop products for deep processing.This research examined the extraction method and application of cyclic adenosine monophosphate(cAMP)from jujube.Using a single-factor experiment,the effects of three parameters on the yield of cAMP were investigated,including solid-liquid ratio,extraction time,and ethanol concentration.The optimal conditions for each ingredient for the highest yield of cAMP were found,and the resulting extract was used to formulate a cream.Using the software Design-Expert V8.0.6 and a central combination design,the experimental group of response surfaces was created.The optimal extraction method for cAMP was determined by establishing a model based on the experimental data,which was 1∶25.42(g∶mL)of the ratio of solids-liquids,63.51 min of extraction time,and 61.63%of the concentration of ethanol,and the cream stability was evaluated.Under the optimal extraction conditions,the yield of cAMP was 189.07μg/g,and a stable cream was formulated with the extract.

关 键 词：红枣 环磷腺苷 响应面 乳霜 稳定性 

分 类 号：TS201.2[轻工技术与工程—食品科学] TQ914.1[轻工技术与工程—食品科学与工程]