灰葡萄孢霉菌产脱落酸发酵工艺优化  

Optimization of fermentation process for abscisic acid production by Botrytis cinerea

作　　者：张俊 左建英 景飞江 陈钢[1] ZHANG Jun;ZUO Jianying;JING Feijiang;CHEN Gang(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Sichuan Lomon Bio Technology Co.Ltd.,Meishan 620020,China)

机构地区：[1]南昌大学,食品科学与技术国家重点实验室,江西南昌330047 [2]四川龙蟒福生科技有限责任公司,四川眉山620020

出　　处：《食品与发酵工业》2024年第2期232-238,共7页Food and Fermentation Industries

基　　金：科技支撑计划“农宝关键技术研发及产业化”(2015NZ0065)。

摘　　要：脱落酸(S-abscisic acid,S-ABA)是五大植物生长调节剂之一,现代农业广泛使用于农业生产,具有增强植物抗逆胁迫,保花保果,促进果实转色、增糖降酸等诸多功能。多种植物病原真菌可以合成,现广泛应用于生产的为灰葡萄孢霉菌(Botrytis cinerea),该文以使用响应面分析方法,考察碳氮比、初始pH、CaCO 3质量浓度、搅拌转速、通气条件、接种量等关键营养条件和工艺条件对Botrytis cinerea菌体形态和产S-ABA的影响。确定最佳发酵条件为:碳氮比25、搅拌强度535 r/min、初始pH 5.1、接种量11%、通气量1.0 vvm,最佳菌丝球尺寸0.95 mm。此条件下S-ABA产量提升了约8.23%。Abscisic acid(S-ABA)is one of the five major plant growth regulators,widely used in modern agriculture.It has many functions such as enhancing plant resistance to stress,preserving flowers and fruits,promoting fruit color change,increasing sugar,and reducing acid.Multiple plant pathogenic fungi can be synthesized,and Botrytis cinerea is widely used for production.This article uses response surface methodology to investigate the effects of key nutritional and process conditions such as carbon nitrogen ratio,initial pH,CaCO 3 content,stirring speed,ventilation conditions,and inoculation amount on the morphology and S-ABA production of B.cinerea.The optimal fermentation conditions were determined as follows:Carbon nitrogen ratio of 25,stirring intensity of 535 r/min,initial pH of 5.1,inoculation amount of 11%,ventilation rate of 1.0 vvm,and optimal mycelium ball size of 0.95 mm.Under this condition,the production of S-ABA increased by approximately 8.23%.

关 键 词：灰葡萄孢霉菌 液态深层发酵 形态学工程 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ452