类胡萝卜素对中华锯齿米虾体色的影响  

The effect of carotenoids on the body color of Neocaridina denticulata sinensis

作　　者：张元长 ZHANG Yuanchang(Zhao an County Aquatic Technology Promotion Station,Zhangzhou 363500,China)

机构地区：[1]诏安县水产技术推广站,福建漳州363500

出　　处：《渔业研究》2024年第1期38-48,共11页Journal of Fisheries Research

基　　金：福建省自然科学基金(2020J01669);国家自然科学基金(31702339)。

摘　　要：为了探究类胡萝卜素对红色、黄色和蓝色3个品系中华锯齿米虾(Neocaridina denticulata sinensis)体色的影响,本实验先通过薄层色谱分析3个品系中华锯齿米虾主要的色素成分,再根据其结果,分别在光照和黑暗条件下进行类胡萝卜素(螺旋藻粉、叶黄素、天然虾青素和人工合成虾青素)对3个品系中华锯齿米虾体色影响的投喂实验。结果表明,红色、黄色、蓝色3个品系中华锯齿米虾的色素物质均含有β-胡萝卜素和虾青素,蓝色品系中华锯齿米虾的色素物质可能含有甲壳蓝蛋白与虾青素的结合物。在光照条件下,天然虾青素对黄色和蓝色2个品系中华锯齿米虾体色的促进效果最明显。在黑暗条件下,叶黄素、天然虾青素和人工合成虾青素对红色和黄色2个品系中华锯齿米虾的体色具有促进和维持效果;4种类胡萝卜素添加剂对蓝色品系中华锯齿米虾的体色均不具有维持效果。本研究结果为提升工厂化养殖中华锯齿米虾体色提供了理论依据。To study the effect of carotenoids on the body color of red,yellow and blue strains of N.denticulata sinensis,this study used TLC(thin-layer chromatography)to analyze the main pigment compositions of 3 strains of N.denticulata sinensis.According to the results,the effects of carotenoids on the body color of 3 strains of N.denticulata sinensis were tested.The TLC analysis was used to separate the dermis and extract the pigment of 3 strains of N.denticulata sinensis.The feeding trials were conducted in light and dark,while 300 shrimps from each strain were divided into thirty groups,with three replicates in each group.The control group was fed with basic feed,while the other four groups were fed with basic feed added Spirulina,lutein,natural-astaxanthin and artificial-astaxanthin.The results showed that the pigment substances of red,yellow,and blue strains of N.denticulata sinensis containedβ-carotene and astaxanthin;the pigment substances of blue N.denticulata sinensis contained a combination of crustacyanin and astaxanthin.The feeding trials showed that in yellow strain and blue strain,natural-astaxanthin had the most obvious effect on the body color promotion.Under the dark condition,lutein,natural-astaxanthin and artificial-astaxanthin had certain promotion and maintenance effects on the body color of red strain and yellow strain.The four carotenoid additives had no effect on the body color in blue strain.This study provided a theoretical basis for improving body color of N.denticulata sinensis.

关 键 词：中华锯齿米虾 类胡萝卜素 Β-胡萝卜素 虾青素 甲壳蓝蛋白 

分 类 号：S968.22[农业科学—水产养殖]