荞麦源性成分实时荧光PCR检测方法的建立  

Establishment of realtime fluorescence PCR detectionmethod for buckwheat derived components

作　　者：王哲贤 张颖[2,3] 亢春雨 曹丽霞[4] 檀建新 赵世峰 WANG Zhe-xian;ZHANG Ying;KANG Chun-yu;CAO Li-xia;TAN Jian-xin;ZHAO Shi-feng(College of Modern Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei,China;College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei,China;Hebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism,Baoding 071000,Hebei,China;Zhangjiakou Academy of Agricultural Sciences,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北农业大学现代科技学院,河北保定071000 [2]河北农业大学食品科技学院,河北保定071000 [3]河北省人畜共患病原微生物分析与防控重点实验室,河北保定071000 [4]张家口市农业科学院,河北张家口075000

出　　处：《粮食与油脂》2024年第2期155-158,162,共5页Cereals & Oils

基　　金：国家重点研发计划项目(2017YFC1601700);2020年河北省引进留学人员资助项目(C20200504)。

摘　　要：为检测荞麦类食品及原料中的荞麦源性成分,根据荞麦品种DW-1核酸序列信息(KY945277.1)设计了荞麦品种特异性引物;以苦荞和甜荞品种基因组为模板,建立了荞麦源性成分的实时荧光PCR(qPCR)的检测方法,并对购自市场的多个粮食产品进行检测,检测结果与商品标识相符方法特异性强、灵敏度高,为食品中荞麦源性成分的检测提供了新思路。In order to detect buckwheat derived components in buckwheat food and raw materials,buck-wheat variety-specific primers were designed according to the DW-1 nucleic acid sequence information of buckwheat variety(KY945277.1).A realtime fluorescence PCR(qPCR)method for the detection of-sweet buckwheat derived components was established using the genome of tartary buckwheat and buck-wheat varieties as templates.Multiple food products purchased from the market were tested,and the de-tection results were consistent with the product labeling.The method has strong specificity and sensitivi-ty,providing a new idea for the detection of buckwheat derived components in food.

关 键 词：荞麦 实时荧光PCR 真实性 过敏原 检测方法 

分 类 号：TS212.7[轻工技术与工程—粮食、油脂及植物蛋白工程]