MMP7和SLPI在甲状腺乳头状癌中的表达及意义  

Expressions of matrix metalloproteinase 7 and secretory leukocyte protease inhibitor in papillary thyroid carcinoma and their significances

作　　者：高峰[1] 蔺广荣 邢立行 李圣陶 赵朋 吕春晖 GAO Feng;LIN Guang-rong;XING Li-hang;LI Sheng-tao;ZHAO Peng;LYU Chunhui(Department of Thyroid Surgery,Binzhou People's Hospital Affiliated to Shandong First Medical University,Binzhou 256610,China)

机构地区：[1]山东第一医科大学附属滨州市人民医院甲状腺外科,山东滨州256610

出　　处：《中国现代普通外科进展》2023年第12期943-946,共4页Chinese Journal of Current Advances in General Surgery

基　　金：滨州市人民医院院级课题(YBKTZR202114)。

摘　　要：目的:探讨基质金属蛋白酶7(MMP7)和分泌型白细胞蛋白酶抑制剂(SLPI)在甲状腺乳头状癌(PTC)中的表达及意义。方法:基于肿瘤基因组图谱(TCGA)数据库中甲状腺癌基因表达数据下载样本567例,包括癌组织509例,正常组织58例。提取基因矩阵数据并整理,利用R语言edge R包筛选出两组差异表达基因,通过Cytoscape中MCODE插件筛选出潜在关键基因20个。选定一个关键基因MMP7,通过UALCAN数据库,挖掘到密切相关的基因SLPI。选取2020年1月至2021年6月山东第一医科大学附属滨州市人民医院收治的69例PTC患者,采用免疫组织化学染色SABC法检测PTC组织和相应癌旁组织中MMP7、SLPI蛋白的表达,分析二者与PTC患者临床病理因素间的关系及二者间的相互关系。结果:MMP7与SLPI存在相关性(r=0.5,P<0.05)。免疫组织化学结果显示,MMP7和SLPI在PTC组织中的阳性表达率均高于癌旁组织[82.6%(57/69)vs 29.0%(20/69),71.0%(49/69)vs15.9%(11/69)],差异均有统计学意义(χ2=40.222、42.579,P均<0.01)。PTC组织中MMP7和SLPI的表达与TNM分期、被膜外侵犯和淋巴结转移均有关(P<0.05)。MMP7和SLPI蛋白在PTC组织中表达呈正相关(r=0.381,P=0.001)。结论:MMP7和SLPI蛋白两者可能相互作用参与PTC的发生与发展。Objective:To investigate the expressions of matrix metalloproteinase 7(MMP7)and secretory leukocyte protease inhibitor(SLPI)in papillary thyroid carcinoma(PTC)and their significances.Methods:Based on the gene expression data of thyroid cancer in the tumor Genome Atlas(TCGA)database,a total of 567 samples were collected,including 509 cancer tissues and 58 normal tissues.The gene matrix data were extracted and sorted out.Two groups of differentially expressed genes were screened by using the R language edger package,and the potential key genes were screened by the mcode plug-in in Cytoscape.Select a key gene and mine closely related genes through the UALCAN database.Immunohistochemical SABC method was used to detect the expressions of MMP7 and SLPI proteins in PTC tissues and their paracancerous tissues collected from 69 patients in Binzhou People's Hospital Affiliated to Shandong First Medical University from January 2020 to June 2021,and the association of expression levels of MMP7 and SLPI with the clinicopathological factors of PTC patients was also analyzed.Results:Based on the data of TCGA database,1471 genes were obtained,of which 1000 were up-regulated and 471 were down-regulated.Through the mcode plug-in in Cytoscape,20 key genes were screened(MMP7,CCL18,CYR61,SPECC1,CRABP2,PLXNA3,KRT17,TMEM59L,RETN,SRF,ITGB4,PPL,PLEKHN1,RMI2,LCN6,SPX,NRIP1,ARHGEF28,SLC39A14,SNCA).Through the UALCAN database,the correlation between MMP7 and SLPI was retrieved(Pearson correlation coefficient was 0.5,P<0.05).The results of immunohistochemistry showed that the positive expression rates of MMP7 and SLPI proteins in PTC tissues were significantly higher than those in paracancerous tissues[82.6%(57/69)vs 29.0%(20/69),71.0%(49/69)vs 15.9%(11/69)],and the differences were statistically significant(χ2 values were 40.222 and 42.579,both P<0.01).The expressions of MMP7 and SLPI in PTC tissues were correlated with TNM stage,differentiation,extramembranous invasion and lymph node metastasis(all P<0.05).There was a positive correlation

关 键 词：甲状腺乳头状癌 肿瘤基因组图谱 计算生物学 基质金属蛋白酶7 分泌型白细胞蛋白酶抑制剂 

分 类 号：R736.1[医药卫生—肿瘤]