Validation of Molecular Detection Methods for Gluten Allergens in Infant Formula  

作　　者：Shuhuan ZHAO Yunxia WANG Cuizhi LI 

机构地区：[1]Inner Mongolia Yili Industrial Group Co.,Ltd.,Inner Mongolia,Hohhot 010110,China

出　　处：《Asian Agricultural Research》2024年第1期29-31,36,共4页亚洲农业研究（英文）

摘　　要：[Objectives]To verify the specificity,sensitivity,precision and negative-positive deviation of the foodproof gluten component de-tection kit for the detection of gluten allergens in milk powder matrix,and to establish a real-time fluorescent PCR legal method for the detec-tion of gluten allergens in milk powder.[Methods]The specificity,sensitivity,precision and negative-positive deviation of the detection method of foodproof gluten component detection kit(PCR-probe method)were verified by artificially adding different concentrations of wheat bran and extracting sample DNA by kit method,and applied to sample detection.[Results] The specific detection results of two kinds of milk powder with wheat bran and buckwheat added showed that the foodproof gluten component detection kit(PCR-probe method)had good speci-ficity for wheat gluten.The results of artificially added wheat bran positive samples showed that the false positive rate and false negative rate of the kit in the milk powder matrix were O,and the sensitivity and precision were high.[Conclusions]The kit is simple to operate and has high accuracy,which is suitable for the detection of gluten allergen components in milk powder.

关 键 词：Real-time fluorescent PCR GLUTEN ALLERGEN Infant formula 

分 类 号：Q93-331[生物学—微生物学]