Characteristics and mechanism of Leuconostoc citreum as a novel bioflocculant for starch granules in starch production  

作　　者：Xuan Wang Pamela Owusu Osei Lei Rao Xiaomeng Wu Xiaojun Liao 

机构地区：[1]College of Food Science and Nutritional Engineering,National Engineering Research Center for Fruit&Vegetable Processing,Key Laboratory of Fruit&Vegetable Processing,Ministry of Agriculture and Agricultural Affairs,Beijing Key Laboratory for Food Non-thermal Processing,China Agricultural University,Beijing 100083,China

出　　处：《Food Innovation and Advances》2023年第4期291-301,共11页食品创新与进展（英文）

基　　金：supported by Sichuan Science and Technology Program 2023NSFSC0181 and the 2115 Talent Development Program of China Agricultural University.

摘　　要：The Leuconostoc citreum SJ-57 strain isolated from the sweet potato starch production showed great potential as a microbiological flocculant,but its underlying flocculation mechanisms are yet unknown.In this study,infrared spectroscopy and thermodynamic analysis were performed to elucidate the short-range and long-range interactions between Leuc.citreum SJ-57 and starch granules,revealing that bacteria cells bond starch granules via metal-bridging ionic bonds.A high repulsive energy barrier of~8×10^(−18)J must be overcome to initiate the flocculation process.Heat,protease,lipase,lysozyme,dextranase,and guanidine hydrochloride were used to treat the bacterial cell,confirming that its flocculation ability originated from surface proteins,including GW structural domain proteins,DnaK,GroEL,elongation factor Tu,and lysozyme M1.The primary flocculation mechanisms of Leuc.citreum was proposed to provide a deep understanding of microbiological flocculants and a foundation for future industrial applications in starch production.

关 键 词：STARCH OVERCOME originated 

分 类 号：TS201[轻工技术与工程—食品科学]