基于铁蛋白纳米笼构建传感元件及其在食品检测中的研究进展  

Research progress of ferritin nanocage-based sensors and their application for detection of food-hazardous substances

作　　者：韩雪儿 谢江 虎梦吉 马良[1,2,3] 郭婷 张宇昊[1,2,3] 尚永彪 陈海[1,2,3] HAN Xueer;XIE Jiang;HU Mengji;MA Liang;GUO Ting;ZHANG Yuhao;SHANG Yongbiao;CHEN Hai(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China;Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]川渝共建特色食品重庆市重点实验室,重庆400715 [3]发光分析和分子传感教育部重点实验室,重庆400715

出　　处：《食品与发酵工业》2024年第3期328-335,共8页Food and Fermentation Industries

基　　金：国家自然科学基金青年项目(32201928);重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX055);十四五国家重点研发计划项目(2021YFD21001005)。

摘　　要：铁蛋白(ferritin)是由24个亚基自组装而成的中空笼形结构蛋白,具有良好的水溶性、稳定性、生物相容性和可逆自组装特性。在生物体内,铁蛋白在铁的吸收、转运、氧化和贮存中扮演重要角色,具有调节体内铁代谢平衡的功能。近年来,由于铁蛋白具有纳米尺度的笼形结构,而且容易通过化学和生物等方法进行结构改造和修饰,使得其被广泛用于检测传感元件的构建。该文对铁蛋白分子的结构与功能进行简要介绍,总结了铁蛋白在构建检测传感元件中的2种基本策略,并重点回顾了基于铁蛋白传感元件在食品真菌毒素、重金属离子、病毒、过氧化氢等有毒有害物质检测中的研究进展,以期为铁蛋白在食品检测领域的应用提供研究思路。Ferritin is composed of 24 subunits that are self-assembled into a cage-like protein nanocage and possess good water solubility,stability,biocompatibility,and reversible self-assembly features.In biology,ferritin proteins play an important role in iron absorption,transport,oxidation,and storage,and participate in iron metabolism.In recent years,due to their nanoscale particle size and robust structure that is amenable to chemical and genetic engineering,ferritin nanocages have been widely used for the construction of biosensors in food,medical,and material sciences.In this paper,by starting with a brief introduction to the structure and function of ferritin nanocages,two basic strategies for the construction of ferritin-based sensors were summarized.Subsequently,the research progress on the ferritin nanocage-based sensors for the detection of food mycotoxins and harmful substances including food toxins,heavy metal ions,viruses,and hydrogen peroxide were reviewed.The purpose of this article was to provide insight into the construction of a ferritin-based sensor for the detection of food hazardous substances.

关 键 词：铁蛋白 传感元件 策略 食品安全 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]