牡蛎肽对帕罗西汀致雄性小鼠性功能障碍的作用效果及潜在机制  

Effects and potential mechanism of oyster peptide on paroxetine-induced sexual dysfunction in male mice

作　　者：黄倩倩 吴海英 秦小明[1,2] HUANG Qianqian;WU Haiying;QIN Xiaoming(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;National Research and Development Branch Center for Shellfish Processing(Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省亚热带果蔬加工科技创新中心,广东湛江524088

出　　处：《南方水产科学》2024年第1期161-172,共12页South China Fisheries Science

基　　金：国家现代农业产业技术体系(CARS-49)。

摘　　要：牡蛎肽(Oyster peptide,OP)具有多种生物活性,然而,其对男性性功能障碍的作用效果仍知之甚少。以牡蛎肽为研究对象,探讨其对男性性功能障碍的作用效果及其潜在机制。每天灌胃帕罗西汀(Paroxetine,PRX)构建雄性小鼠性功能障碍模型,同时灌胃牡蛎肽(500 mg·kg^(−1)),持续28 d。结果表明:与模型(PRX)组小鼠相比,牡蛎肽可显著提高雄性小鼠的性能力(P<0.05),恢复血清性激素水平(P<0.01),提高阴茎组织一氧化氮(NO)含量(P<0.01)、环磷酸鸟苷(cGMP)含量(P<0.05),和一氧化氮合酶(NOS)活性(P<0.05),并降低磷酸二酯酶-5(PDE-5)活性(P<0.01);同时,牡蛎肽可增强睾丸标志性酶活性(P<0.05)和抗氧化能力(P<0.01),改善精子质量。此外,HE染色结果显示:牡蛎肽可恢复小鼠睾丸生精小管内生精细胞的数量与形态,减少生精小管空泡化现象。综上所述,牡蛎肽可有效减缓PRX导致的雄性小鼠性功能障碍,推测其对男性性功能障碍具有潜在的保护作用。Oyster peptide(OP)has various biological activities.However,its effects on male sexual dysfunction is still poorly understood.In this study,we explored its effects and potential mechanism on male sexual dysfunction.Besides,we established a paroxetine(PRX)-induced sexual dysfunction model by gavaging OP(500 mg·kg^(−1))in mice for 28 d.The results show that compared with the model(PRX)group,OP could improve the sexual performance of male mice(P<0.05),restored serum sex hormone levels(P<0.01),increased penile tissue nitric oxide(NO)content(P<0.01),cyclic guanosine monophosphate(cGMP)content(P<0.05)and nitric oxide synthase(NOS)activity(P<0.05),and decreased phosphodiesterase-5(PDE-5)activity(P<0.01).Meanwhile,OP enhanced testicular marker enzymes activities(P<0.05)and antioxidant capacity(P<0.01),and improved sperm quality.In addition,HE staining results show that OP could restore the number and morphology of spermatogenic cells in seminiferous tubules of mice,and reduced the vacuolization of seminiferous tubules.In conclusion,OP can alleviate PRX-induced sexual dysfunction effectively in male mice and has a potential protective effect on male sexual dysfunction.

关 键 词：牡蛎肽 帕罗西汀 性功能障碍 勃起障碍 性行为 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]