4-氰基吡啶合成异烟酸菌种发酵工艺的研究  

Study on the Fermentation Technology of Isonicotinic Acid Strain for Synthesis of 4-Cyanopyridine

作　　者：周浩 齐勇 刘淑琦 陈洋 ZHOU Hao;QI Yong;LIU Shu-qi;CHEN Yang(Anhui Ruibang Biotechnology Co.Ltd.,Maanshan 243100,China)

机构地区：[1]安徽瑞邦生物科技有限公司,安徽马鞍山243100

出　　处：《安徽化工》2023年第6期81-84,89,共5页Anhui Chemical Industry

摘　　要：简单介绍了一株4-氰基吡啶合成异烟酸的菌种发酵工艺的研究,探究了不同碳源、氮源、诱导剂浓度、金属离子种类及金属离子浓度对发酵产腈水解酶酶活的影响,并对菌种高密度发酵工艺做了一系列优化,优化后的培养基对于该菌产腈水解酶的酶活达到了1076 U/mL,OD600达到了185,酶活对比优化前提高了40%。该菌在催化4-氰基吡啶合成异烟酸行业具有重要意义。This paper briefly introduces the fermentation process of a strain of 4-cyanopyridine to synthesize isonicotinic acid,explores different carbon sources,nitrogen sources,inducer concentrations,metal ion types and metal ion concentrations,and makes a series of optimization of the above conditions on the high density fermentation of the strain.The optimized medium for the bacteria to produce nitrile-hydrolase enzyme activity reached 1076 U/mL,OD600 reached 185,and enzyme activity increased by 40% compared with before optimization.This bacterium is of great significance in the industry of catalyzing the synthesis of 4-cyanopyridine isoniacin.

关 键 词：4-氰基吡啶 异烟酸 腈水解酶 发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]