黑豆-乳清双蛋白膳食对大鼠体内免疫的调节作用  

Effects of Black Soybean-whey Protein Blend on Immune Regulation in Rats

作　　者：梁得福 孟维洪 舒欣 庄柯瑾 张东杰[1,3] LIANG Defu;MENG Weihong;SHU Xin;ZHUANG Kejin;ZHANG Dongjie(Heilongjiang Bayi Agricultural University,College of Food Science and Technology,Daqing 163319,China;Quality Supervision,Inspection and Testing Center of Agricultural Products and Processed Products,Ministry of Agriculture and Rural Affairs,Daqing 163319,China;Heilongjiang Key Laboratory of Agricultural Products Processing and Quality Safety,National Coarse Grain Engineering Research and Technology Center,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]农业农村部农产品及加工品质量监督检验测试中心,黑龙江大庆163319 [3]黑龙江省农产品加工与质量安全重点实验室,国家杂粮工程研究技术中心,黑龙江大庆163319

出　　处：《现代食品科技》2024年第1期1-9,共9页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2018YEE0206300);黑龙江省博士后面上资助项目(LBH-Z20205);黑龙江八一农垦大学“三纵”基础培育计划(ZRCPY202004)。

摘　　要：黑豆蛋白和乳清蛋白分别是两种优质的植物源和动物源蛋白质原料,食用后均可改善机体的免疫特性,但双蛋白对机体免疫调节作用的研究相对较少。本试验利用标准膳食(CP)、乳清蛋白膳食(WP)、黑豆蛋白膳食(BSP)和黑豆-乳清双蛋白膳食(BS-WP)对4组大鼠进行为期60 d的喂养,喂养周期结束后对各组大鼠的血清生化指标、免疫球蛋白(Immunoglobulin,Ig)IgM、IgA和IgG质量浓度、脾淋巴细胞增殖指数、自然杀伤细胞活力、脾脏指数和胸腺指数进行测定,探究黑豆乳清双蛋白对大鼠体内免疫的调节作用。结果表明,与单一蛋白源相比,BS-WP组大鼠的血清免疫生化指标总蛋白、白蛋白、球蛋白、尿素氮、和谷丙转氨酶显著提升(P<0.05);IgG、IgA和IgM的质量浓度均有显著增加(P<0.05),且IgG增加最为显著,与单一WP组和BSP组相比,BS-WP组大鼠的IgG分别增加了38.23%和41.02%,此外,脾脏和胸腺指数也均有显著增加(P<0.05),脾脏指数分别增加了35.33%和35.91%,胸腺指数分别增加了31.51%和34.26%。由此可知,黑豆-乳清双蛋白膳食可显著提升机体免疫能力。Black soybean protein and whey protein are two kinds of high-quality plant or animal protein materials.Their consumption can improve the immune characteristics of the body.However,there are relatively few studies on the immunomodulatory effect of the black soybean-whey protein blend.In this study,four groups of rats were fed with a standard diet(CP),whey protein diet(WP),black bean protein diet(BSP)and black soybean-whey protein blend diet(BS-WP)for 60 days.At the end of the feeding cycle,serum biochemical indicators,IgM,IgA and IgG mass concentrations,spleen lymphocyte proliferation index,natural killer cell activity,spleen index and thymus index were measured to explore the regulatory effect of black soybean-whey protein blend on the immune system of rats.Results showed that compared with the single protein source group,the serum immune biochemical indexes(total protein,albumin,globulin,urea nitrogen,alanine transaminase levels)of the black soybean-whey protein blend group significantly increased(P<0.05).The mass concentrations of IgG,IgA and IgM increased significantly(P<0.05),with the increase of IgG content being the most significant.Compared with the groups treated whey protein or black soybean protein singly,the IgG content of the protein blend group increased by 38.23%and 41.02%,respectively.In addition,the spleen and thymus indexes significantly increased(P<0.05),with the increase of the spleen index being 35.33%and 35.91%,respectively,and the increase of the thymus index being 31.51%and 34.26%,respectively.Therefore,black soybean-whey blended protein blend diet can significantly improve the body immunity capacities of rats.

关 键 词：黑豆蛋白 乳清蛋白 黑豆-乳清双蛋白 免疫调节 

分 类 号：TS201.4[轻工技术与工程—食品科学]