芽孢杆菌B55产羧甲基纤维素酶的培养优化研究  

Culture Optimization of Bacillus sp.B55 for the Production of Carboxymethyl-cellulase

作　　者：张佳雯 王玉芳 张忠民 卢宽 鲁仪增 程仕伟[1] ZHANG Jiawen;WANG Yufang;ZHANG Zhongmin;LU Kuan;LU Yizeng;CHENG Shiwei(Yantai Engineering Laboratory of Biological Fermentation and Separation,School of Life Sciences,School of Life Sciences,Ludong University,Yantai 264025,China;Yantai Vacational College,Yantai 264670,China;Shandong Forest and Grass Germplasm Resources Center,Jinan 250102,China)

机构地区：[1]鲁东大学生命科学学院烟台市生物发酵与分离工程实验室,山东烟台264025 [2]烟台职业学院,山东烟台264670 [3]山东省林草种质资源中心,山东济南250102

出　　处：《工业微生物》2024年第1期76-80,共5页Industrial Microbiology

基　　金：烟台市科技计划发展项目(编号:2022XDRH029);山东省重点研发计划(重大科技创新工程)项目(编号:2021LZGC02304)。

摘　　要：芽孢杆菌来源的纤维素酶具有耐热耐碱等优良催化特性,故而受到了广泛关注。本研究利用筛选的芽孢杆菌B55发酵生产羧甲基纤维素酶,在确定碳源、氮源、金属盐、羧甲基纤维素添加量、接种量等对菌株产酶影响的基础上,进行正交试验优化以确定最佳发酵培养工艺。研究结果表明:最佳培养工艺的配方为30 g/L甘油、5 g/L葡萄糖、3 g/L大豆蛋白胨、16 g/L酵母浸粉、4 g/L羧甲基纤维素、3 g/L磷酸氢二钾,3 g/L磷酸二氢钠,pH 7.0,在4%(V/V)接种量、培养温度32℃的条件下,发酵48 h获得最大酶活力401.34 U/mL,具备规模生产的应用潜力。Cellulase from Bacillus has excellent catalytic properties such as heat and alkali resistance,so it has been attracted wide attention.In this paper,carboxymethyl cellulase(CMCase)was produced with the screened Bacillus sp.B55.On the basis of determining the effect of carbon source,nitrogen source,metal salt,carboxymethyl cellulose addition and inoculation amount on the CMCase production of the strain B55,the fermentation cultural process was further optimized by orthogonal experiment design.The results indicated the optimal medium was 30 g/L glycerine,5 g/L glucose,3 g/L soybean peptone,16 g/L yeast powder,4 g/L CMC,3 g/L K2HPO_(4),3 g/L NaH_(2)PO_(4),pH 7.0,and then 401.34 U/mL of the maximum CM Case activity was obtained at 48 h under the conditions of 4%(V/V)inoculums and culture temperature of 32℃,so which had potential for scale production and application.

关 键 词：羧甲基纤维素酶 芽孢杆菌 培养优化 发酵生产 

分 类 号：TQ925[轻工技术与工程—发酵工程]