鸡矢藤叶绿体基因组分析  

Analysis on Chloroplast Genome of Paederia scandens

作　　者：吴民华 吴子健 叶晓霞 谭靖怡 王燊 黄琼林 WU Minhua;WU Zijian;YE Xiaoxia;TAN Jingyi;WANG Shen;HUANG Qionglin(School of Basic Medicine,Guangdong Medical University,Zhanjiang 524023,China;The First Clinic Medical College,Guangdong Medical University,Zhanjiang 524023,China;School of Pharmacy,Guangdong Medical University,Zhanjiang 524023,China)

机构地区：[1]广东医科大学基础医学院,广东湛江524023 [2]广东医科大学第一临床医学院,广东湛江524023 [3]广东医科大学药学院,广东湛江524023

出　　处：《河南农业科学》2024年第1期70-77,共8页Journal of Henan Agricultural Sciences

基　　金：广东省基础与应用基础研究基金项目(2018A030310116);广东省大学生创新创业训练计划项目(S202310571113);广东医科大学大学生创新创业训练计划项目(GDMU2022113,GDMU2023224)。

摘　　要：为了阐明岭南特色药用植物鸡矢藤的叶绿体基因组序列特征,采用高通量测序技术开展鸡血藤叶绿体基因组测序,再借助生物信息学方法对测得序列进行拼接、注释和比对分析。结果表明,鸡矢藤叶绿体基因组为153456 bp的环状双链四分体结构,共编码133个基因。在鸡矢藤叶绿体基因组中发现54个简单重复序列,其中A/T单核苷酸重复最多,占比61.1%;共找到26983个密码子,第3位碱基是A/T的密码子具有更高的使用频次。序列比对分析显示,鸡矢藤等5种茜草科植物在基因间隔区和内含子等非编码区域存在显著的碱基突变。系统进化树清晰地展现了鸡矢藤在茜草科内的进化位置,且不同来源的鸡矢藤序列同源性高。To elucidate the structure and sequence characteristics of chloroplast genome from Paederia scandens,the chloroplast genome sequencing was performed using high-throughput sequencing technology,and then acquired sequence was assembled,annotated and analyzed via bioinformatics procedures.The results demonstrated that the chloroplast genome of P.scandens was a circular quadripartite molecule with a length of 153456 bp and encoded 133 genes.Altogether 54 simple sequence repeats were detected in the plastome and the A/T mononucleotide was main type.A total of 26983 codons were searched out and those with end of A/T were frequently used.Sequence alignment demonstrated more divergence in non-coding region among five Rubiaceae species including P.scandens.Phylogenetic tree revealed close relationship of various chloroplast genome sequences of P.scandens and clearly displayed the evolutionary position of P.scandens in Rubiaceae.

关 键 词：鸡矢藤 叶绿体基因组 序列特点 序列比对 系统进化分析 

分 类 号：S567.239[农业科学—中草药栽培]