海水曝气对海洋微生物群落和抗生素抗性基因的影响  

Effects of Seawater Aeration on Marine Microbial Communities and Anti-biotic Resistance Genes

作　　者：林旭吟[1,4] 王艳 许敏 张振炎 钱海丰 Lin Xuyin;Wang Yan;Xu Min;Zhang Zhenyan;Qian Haifeng(College of Marine Biology,Xiamen Ocean Vocation College,Xiamen 361100,China;College of Environment,Zhejiang University of Technology,Hangzhou 310032,China;Moganshan Institute,Zhejiang University of Technology,Huzhou 310000,China;Xiamen Key Laboratory of Intelligent Fishery,XiaMen Ocean Vocation College,Xiamen 361100,China)

机构地区：[1]厦门海洋职业技术学院海洋生物学院,厦门361100 [2]浙江工业大学环境学院,杭州310032 [3]浙江工业大学莫干山研究院,湖州310000 [4]厦门市智慧渔业重点实验室,厦门海洋职业技术学院,厦门361100

出　　处：《生态毒理学报》2024年第1期252-261,共10页Asian Journal of Ecotoxicology

基　　金：厦门市智慧渔业重点实验室开放基金;福建海洋生物应用技术协同创新中心专项(XTZX-HYSW-1804);福建省中青年教师教育科研项目(JAT191318)。

摘　　要：占地球表面71%的海洋蕴含丰富且独特的微生物资源。为维持海产品的鲜活,人们常采用循环暂养技术,通过曝气装置增加水体含氧量。本研究运用宏基因组技术,分析氧含量变化对海洋微生物群落结构、功能组成及抗生素抗性基因的影响。研究发现,高氧海水中的以α-变形菌纲为主的微生物群落多样性较高,而微生物群落功能多样性则随着氧浓度升高而降低。海洋微生物中多种抗生素抗性基因与毒力因子基因丰度下降。这表明海水中氧含量的上升可以减少微生物的抗药性和致病潜能,从而降低其对人类健康的潜在风险。Covering 71%of the Earth’s surface,the oceans harbor abundant and distinct microbial resources.To preserve the freshness of seafood,cyclic temporary breeding technique is commonly used to elevate oxygen level in water.Our study employed metagenomic technique to analyze the influence of oxygen level on the structure,functional traits,and antibiotic resistance genes of microbial communities in marine.Results indicated that marine microbial community was dominated by Alphaproteobacteria and exhibited higher diversity in high-oxygen level than which in low level.However,functional diversity of microbial communities decreased with the rising oxygen level.The abundances of various antibiotic resistance genes and virulence factor genes declined significantly with increasing oxygen concentration.These results suggested that the rise of oxygen level in seawater can reduce microbial resistance to antibiotics and their pathogenicity,thereby mitigate potential health risks to humans.

关 键 词：抗生素抗性基因 海洋微生物 毒力因子 高氧处理 

分 类 号：X171.5[环境科学与工程—环境科学]