Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance  

作　　者：Haofei Luo Xiaofei Wang Changqing You Xuedan Wu Duofeng Pan Zhiyao Lv Tong Li Dongmei Zhang Zhongbao Shen Xiaodong Zhang Guodao Liu Kaixuan He Qingtong Ye Yajun Jia Qinghua Zhao Xian Deng Xiaofeng Cao Xianwei Song Gai Huang 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]Hainan Yazhou Bay Seed Lab,Sanya 572025,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Institute of Forage and Grassland Sciences,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China [5]Shandong Academy of Agricultural Sciences,Jinan 250100,China [6]National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land,Dongying 257345,China [7]Shandong Green Manure Ecological Technology Co.,Ltd,Dongying 257345,China [8]State Key Laboratory of Tropical Crop Breeding,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

出　　处：《Science China(Life Sciences)》2024年第1期149-160,共12页中国科学（生命科学英文版）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030000);the National Key Research and Development Program of China(2022YFD1500503,2022YFF1003401);Science&Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta(2022SZX14);the earmarked fund for CARS-Green Manure(CARS-22);the Youth Innovation Promotion Association of CAS(Y2022039).

摘　　要：Alkaline soils pose an increasing problem for agriculture worldwide,but using stress-tolerant plants as green manure can improve marginal land.Here,we show that the legume Sesbania cannabina is very tolerant to alkaline conditions and,when used as a green manure,substantially improves alkaline soil.To understand genome evolution and the mechanisms of stress tolerance in this allotetraploid legume,we generated the first telomere-to-telomere genome assembly of S.cannabina spanning~2,087 Mb.The assembly included all centromeric regions,which contain centromeric satellite repeats,and complete chromosome ends with telomeric characteristics.Further genome analysis distinguished A and B subgenomes,which diverged approximately 7.9 million years ago.Comparative genomic analysis revealed that the chromosome homoeologs underwent large-scale inversion events(>10 Mb)and a significant,transposon-driven size expansion of the chromosome 5A homoeolog.We further identified four specific alkali-induced phosphate transporter genes in S.cannabina;these may function in alkali tolerance by relieving the deficiency in available phosphorus in alkaline soil.Our work highlights the significance of S.cannabina as a green tool to improve marginal lands and sheds light on subgenome evolution and adaptation to alkaline soils.

关 键 词：SESBANIA cannabina alkaline stress telomere-to-telomere GENOME LEGUME green manure phosphate TRANSPORTER ALLOTETRAPLOID 

分 类 号：Q943.2[生物学—植物学]