食叶草多酚类物质的提取、精制工艺研究  

Extraction and refining technology of polyphenols from edible dock

作　　者：左梦微 任顺成[1] 王凤成[1] ZUO Mengwei;REN Shuncheng;WANG Fengcheng(Henan Provincial Key Laboratory of Natural Pigment Preparation,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学河南省天然色素制备重点实验室,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2023年第6期36-43,共8页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南省重点研发专项(221111112000);河南工业大学自科创新基金支持计划(2022ZKCJ16)。

摘　　要：为探究食叶草多酚类物质的潜在价值,通过单因素和响应面试验,对食叶草多酚类物质的提取工艺进行优化,并依次采用大孔吸附树脂、聚酰胺对其进行富集和精制。结果表明:当提取温度39℃、料液比1∶33 g·mL^(-1)、提取液乙醇体积分数为78%时,食叶草多酚类物质得率最高,达到(22.73±0.13)mg·g^(-1),提取液冻干后测得粗提物的多酚类物质纯度为7.20%±0.51%;通过对不同极性的大孔树脂进行筛选,发现AB-8型树脂吸附、解吸效果最好,采用该树脂对食叶草多酚类物质富集后,多酚类物质纯度达到34.28%±2.86%;进一步经过聚酰胺精制,用体积分数40%乙醇为洗脱液对其进行洗脱,多酚类物质纯度最高达到53.57%±4.31%,与粗提物相比,食叶草多酚类物质纯度提高了7.3倍。高效液相色谱分析表明大孔吸附树脂和聚酰胺对食叶草多酚具有较好的富集、精制和粗分效果。本研究优化了食叶草多酚类物质提取工艺,有效实现多酚类物质的精制,为拓展食叶草的应用提供了参考依据。To explore the potential value of polyphenols in edible dock,in this study,single-factor and response surface experiments were carried out to optimize the extraction of polyphenols from edible dock,and macroporous adsorption resin and polyamide were used to enrich and refine them.The results showed that when the extraction temperature was 39℃,the ratio of solid to liquid was 1∶33 g·mL^(-1),and the volume fraction of ethanol was 78%,the content of polyphenols was the highest,which could reach(22.73±2.73)mg/g.After freeze-drying,the polyphenol purity of the crude extract was 7.20%±0.51%.2r freeze-drying,themacroporous resins with different polarities,By screening of macroporous resins of different polarity,it was found that AB-8 resin had the best adsorption and desorption effect.After the enrichment of polyphenols by this resin,the purity of polyphenols reached 34.28%±2.86%,it was found that AB-8 resin had the best adsorption and desorption effect.After the enrichment of polwas the polyphenols was further refined and eluted with 40%ethanol.The highest purity of polyphenols was 53.57%±4.31%.Compared with the crude extract,the purity of polyphenols was nearly 7.3 times higher.Further analysis by high performance liquid chromatography(HPLC)showed that the macroporous adsorbent resin and polyamide had good enrichment,refining and crude separation effects on the polyphenols of the edible dock.In this study,the extraction process of polyphenols was optimized,and the refining of polyphenols was realized effectively,which provided a reference for expanding the application of the edible dock.

关 键 词：食叶草 植物多酚 大孔树脂 聚酰胺树脂 

分 类 号：TS201.2[轻工技术与工程—食品科学]