柚皮苷制备柚皮苷二氢查尔酮的氢化工艺优化  

Optimization of hydrogenation process for preparation of naringin dihydrochalcone from naringin

作　　者：黄凯 毛子剑 刘学魁 倪辉[1,3,4] 李利君 李清彪[1,3,4] HUANG Kai;MAO Zijian;LIU Xuekui;NI Hui;LI Lijun;LI Qingbiao(College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021;Xiamen Oamic Biotech Co.,Ltd.,Xiamen 361026;Fujian Provincial Key Laboratory of FoodMicrobiology and Enzyme Engineering,Xiamen 361021;Xiamen Research Center ofFood Biotechnology,Xiamen 361021)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021 [2]厦门欧米克生物科技有限公司,厦门361026 [3]福建省食品微生物与酶工程重点实验室,厦门361021 [4]厦门市食品生物工程技术研究中心,厦门361021

出　　处：《中国食品添加剂》2024年第2期48-57,共10页China Food Additives

基　　金：国家自然科学基金(U1805235)。

摘　　要：为探讨柚皮苷二氢查尔酮的制备合成工艺,本文通过单因素试验和响应面试验优化制备过程中的氢化工艺,并对氢化产物进行纯化鉴定研究。得到优化氢化工艺为:反应温度25℃、反应压力0.5 MPa、柚皮苷添加量5.8 g/L、NaOH用量为0.21%、反应溶剂为水、反应时间为7.90 h、催化剂为10%的Pd/C、催化剂用量为0.16%。在此条件下,柚皮苷二氢查尔酮的合成产率为93.11%。对合成产物进行结晶纯化,结晶产物经HPLC分析以及核磁共振技术鉴定为柚皮苷二氢查尔酮,纯度为92%。本研究的优化工艺具有反应温度低、反应压力小、反应时间短、柚皮苷二氢查尔酮产率高、产物容易纯化制备等优点,为工业化生产柚皮苷二氢查尔酮提供了高效的氢化工艺参考。In order to investigate the preparation and synthesis process of naringin dihydrochalcone,the hydrogenation process in the preparation process was optimized by single factor test and response surface test,and the hydrogenated products were purified and identified.The optimum hydrogenation process was as follows:at 25℃and a pressure of 0.5 MPa,the addition of naringin was 5.8 g/L,the amount of NaOH was 0.21%,the reaction solvent was water,the reaction time was 7.90 h,the catalyst was 10%palladium carbon(Pd/C),and the catalyst amount was 0.16%.Under these conditions,the synthesis yield of naringin dihydrochalcone was 93.11%.After crystallization,the synthesized product was identified by HPLC and nuclear magnetic resonance techniques,and the product was determined to be naringin dihydrochalcone,and the purity was 92%.The process had the advantages of low reaction temperature,small reaction pressure,short reaction time,high yield of naringin dihydrochalcone,and easy purification and preparation of the product.The study provides reference for an efficient hydrogenation and refining process for industrial production of naringin dihydrochalcone.

关 键 词：柚皮苷 柚皮苷二氢查尔酮 氢化 钯碳 响应面 

分 类 号：TS202.3[轻工技术与工程—食品科学]