酱渣大豆异黄酮大孔树脂纯化工艺及其抗氧化活性研究  

Study on Purification Process of Soybean Isoflavones from Sauce Residue by Macroporous Resin and Its Antioxidant Activity

作　　者：唐银 陈婧司 沈子绮 何贵萍[1] 张佳琪[1] 吕远平[1] TANG Yin;CHEN Jing-si;SHEN Zi-qi;HE Gui-ping;ZHANG Jia-qi;LYU Yuan-ping(College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China;School of Public Health,Suzhou Medical College of Soochow University,Suzhou 215123,China)

机构地区：[1]四川大学轻工科学与工程学院,成都610065 [2]苏州大学苏州医学院公共卫生学院,江苏苏州215123

出　　处：《中国调味品》2024年第3期61-67,共7页China Condiment

基　　金：2021年度四川大学-泸州市人民政府战略合作项目(2021CDLZ-18)。

摘　　要：研究酱渣大豆异黄酮的纯化工艺及其抗氧化活性。选用5种树脂(AB-8、HPD300、ADS-7、DM301、D101)进行静态吸附试验,筛选吸附效果最好的树脂,再以动态吸附试验筛选最佳纯化工艺,最后研究纯化后酱渣大豆异黄酮的抗氧化活性。结果表明,AB-8树脂的吸附效果最好,在流速1.5 mL/min、pH 3的条件下吸附0.6 mg/mL的样品溶液75 mL,再用100 mL 60%乙醇溶液解吸,样品纯度可由11.37%提升到55.84%;纯化后的酱渣大豆异黄酮具有较好的抗氧化活性,其浓度为0.07 mg/mL时,对DPPH自由基和ABTS自由基的清除率分别为93.35%和98.67%。AB-8树脂可用于酱渣大豆异黄酮的分离纯化且效果较好,酱渣大豆异黄酮具有较强的抗氧化活性。The purification process and antioxidant activity of soybean isoflavones from sauce residue are studied.Five resins(AB-8,HPD300,ADS-7,DM301 and D101)are selected for static adsorption test to screen the resin with the best adsorption effect,and then the optimal purification process is screened by dynamic adsorption test.Finally,the antioxidant activity of soybean isoflavones from sauce residue after purification is studied.The results show that AB-8 resin has the best adsorption effect.Under the conditions of the flow rate of 1.5 mL/min and pH 3,75 mL 0.6 mg/mL sample solution is adsorbed,and then desorbed with 100 mL 60% ethanol solution.The purity of the sample can increase from 11.37% to 55.84%.The purified soybean isoflavones from sauce residue have good antioxidant activity.When its concentration is 0.07 mg/mL,the scavenging rates on DPPH radicals and ABTS radicals are 93.35% and 98.67% respectively.AB-8 resin can be used for the separation and purification of soybean isoflavones from sauce residue with good effect,and soybean isoflavones from sauce residue have strong antioxidant activity.

关 键 词：酱渣 大豆异黄酮 大孔树脂 纯化 抗氧化 

分 类 号：TS264.24[轻工技术与工程—发酵工程]