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作 者:傅佳蕊 刘世林 牛泽岳 郑丽鋆 徐会有[1] 倪林[1] FU Jiarui;LIU Shilin;NIU Zeyue;ZHENG Lijun;XU Huiyou;NI Lin(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Department of Pharmacy,Fujian Medical University Union Hospital,Fuzhou,Fujian 350001,China)
机构地区:[1]福建农林大学植物保护学院,福建福州350002 [2]福建医科大学附属协和医院药学部,福建福州350001
出 处:《生物安全学报(中英文)》2024年第1期77-82,共6页Journal of biosafety
基 金:福建省自然科学基金面上项目(2022J01244);福建省本科高校研究生教研重大项目(FJBKJY20220170)。
摘 要:【目的】研究独蒜兰假鳞茎乙醇提取物对植物病原真菌的抑菌作用,为植物源杀菌剂的开发提供依据。【方法】采用菌丝生长速率法,研究独蒜兰假鳞茎乙醇提取物对15种植物病原菌的抑制活性;以西瓜尖孢镰刀菌作为供试菌,进一步研究该提取物对病原真菌的菌丝干重、细胞膜、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)等的影响。【结果】独蒜兰假鳞茎提取物对辣椒疫霉病菌、西瓜尖孢镰刀菌、番茄灰霉病菌和非洲隐地疫霉菌的抑菌效果明显,其EC_(50)值分别为0.849、0.782、0.813和1.161 mg·mL^(-1);经独蒜兰提取物处理后的西瓜尖孢镰刀菌菌丝干重随着药剂浓度的增加而减少;细胞膜丙二醛含量和相对电导率增加;菌丝体细胞内CAT、POD和SOD 3种保护酶活性增加。【结论】独蒜兰假鳞茎提取物对植物病原真菌具有较好的抑菌活性,其抑菌作用可能与其干扰菌丝生长、使菌丝细胞膜正常功能受损等有关。【Aim】This study aimed to investigate the inhibitory effects of Pleione bulbocodioides(Franch.)Rolfe ethanol extracts on plant pathogenic fungi and to provide a basis for the development of plant-derived fungicides.【Method】The mycelial growth rate method was used to investigate the inhibitory activity of the extract against 15 plant pathogenic fungi.The effects of the extract on the mycelial dry weight,cell membrane,catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)activities of pathogenic fungi were further studied using the fungus Fusarium oxysporumf as the test strain.【Result】The P.bulbocodioides extract showed significant inhibitory effects on Phytophthora capsici,Fusarium oxysporum,Botrytis cinerea,and Phytophthora cryptogea,with EC_(50) values of 0.849,0.782,0.813,and 1.161 mg·mL^(-1),respectively.The mycelial dry weight of F.oxysporumf decreased with increasing extract concentrations.The malondialdehyde content of the cell membrane and relative conductivity increased.The activities of CAT,POD,and SOD,three protective enzymes in mycelia,increased.【Conclusion】Extracts from the pseudobulb of P.bulbocodioides had good inhibitory activity against plant pathogenic fungi.This inhibitory effect may be related to its interference with mycelial growth and damage to the normal functions of the mycelial cell membrane.
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