复合酶改性玉米皮可溶性膳食纤维的工艺优化及理化特性分析  

Process Optimization and Physicochemical Characteristics Analysis of Soluble Dietary Fiber from Corn Bran Modified by Composite Enzymes

作　　者：魏旭瑶 姜彩霞 曾祥瑞 王萌 郑喜群 刘晓兰 WEI Xuyao;JIANG Caixia;ZENG Xiangrui;WANG Meng;ZHENG Xiqun;LIU Xiaolan(College of Food Science,Heilongjiang Bayi Agricultural Unive rsity,Daqing 163319,China;National Coarse Cereals Engineering Research Center,Heilongji ang Bayi Agricultural University,Daqing 163319,China;Engineering Research Center of Processing and Utilization of Grain By-products and Utilization of Ministry of Education,Daqing 163319,China;College of Food and Biological Engineering,Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319 [4]齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品工业科技》2024年第6期202-209,共8页Science and Technology of Food Industry

基　　金：黑龙江省百千万工程科技重大专项子课题(2021ZX12B09-4);国家十四五重点研发计划子课题(2021YFD2100904)。

摘　　要：为探究玉米皮可溶性膳食纤维(Soluble Dietary Fiber,SDF)最佳提取工艺及功能特性。本研究以玉米加工副产物玉米皮为原料,采用复合酶法改性提取玉米皮可溶性膳食纤维,通过单因素实验和响应面优化确定最佳工艺参数并对其理化性质进行了研究。结果表明,当纤维素酶添加量为1.5%、木聚糖酶添加量为1%、酶解温度为55℃、酶解时间为150 min时,玉米皮SDF得率达到的最高值为16.64%±0.21%,其溶解度为87.63%±0.43%,持水力为2.87±0.16 g/g,持油力为2.30±0.12 g/g,葡萄糖吸附力为5.32±0.12 mmol/g,体外模拟胃环境(pH2.0)、肠环境(pH7.0)下胆固醇吸附性分别为11.74±0.15、42.93±0.08 mg/g,胆酸钠吸附性分别为15.43±0.17、50.67±0.10 mg/g。研究结果可为玉米皮可溶性膳食纤维的开发利用提供理论参考。To investigate the optimal extraction process and functional c haracteristics of soluble dietary fiber(SDF)from corn bran.This study used corn bran,a by-product of corn processing,as raw material to extract solubl e dietary fiber from corn bran through composite enzyme modification.The optimal p rocess parameters were determined through single factor experiments and response surface methodology optimization,and their physicochemical properties were studied.The results showed that the extraction yield of SDF reached the highest value of 16.64%±0.21%when the content of cellulase was 1.5%,the content of xylanase was 1%,the enzymolysis temperature was 55℃and enzymolysis time was 150 min.Its solubility was 87.63%±0.43%,the holding water capacity was 2.87±0.16 g/g,the o il holding capacity was 2.30±0.12 g/g,the glucose adsorption capacity was 5.32±0.12 mmol/g.Under simulated stomach(pH2.0)and intestinal environment(pH7.0),cholesterol adsorption capacity was 11.74±0.15 and 42.93±0.08 mg/g and sodium cholacte adsorption capacity was 15.43±0.17 and 50.67±0.10 mg/g,respectively.The research re sults can provide theoretical reference for the development and utilization of soluble dietary fiber from corn bran.

关 键 词：玉米皮 可溶性膳食纤维 复合酶改性 响应面优化 理化性质 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]