产细菌素Enterocin Y3粪肠球菌对小鼠肠道菌群及代谢功能的影响  

Effects of an Enterocin Y3 Producing Strain of Enterococcus faecalis DH9003 on Intestinal Flora Modification and Metabolic Function of Mice

作　　者：杨珍珠 陶乐仁[2] 赵陆恺 王元[1] 迟海[1,2] Yang Zhenzhu;Tao Leren;Zhao Lukai;Wang Yuan;Chi Hai(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090;School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海理工大学健康科学与工程学院,上海200093

出　　处：《中国食品学报》2024年第2期99-109,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：上海市自然科学基金项目(22ZR1478500);中央级公益性科研院所基本科研业务费专项资金(2016M02)。

摘　　要：为探讨产细菌素Enterocin Y3粪肠球菌DH9003的益生菌作用,对15只健康C57雄性小鼠进行等体积灌胃无菌生理盐水(对照组,n=7)和粪肠球菌DH9003(处理组,n=8),4周后收集小鼠粪便,测定粪肠球菌DH9003对小鼠肠道菌群结构和代谢功能的影响。结果显示,用粪肠球菌DH9003灌胃小鼠,7 d内就可在其体内大量定植;7~28 d时,该细菌数量缓慢增加并趋近稳定。通过观察小鼠肠道和肝脏组织切片研究病理学特征,灌胃粪肠球菌DH9003不会对小鼠肠道和肝脏产生有害影响,相反会使小鼠的肠黏膜更为密集且长度增加,改善小鼠肠黏膜。用粪肠球菌DH9003灌胃使该菌株成为不同分组小鼠肠道差异的主要物种。同时,补充粪肠球菌DH9003对小鼠肠道的菌群结构具有一定的调节作用,导致门水平上的变化为拟杆菌门和厚壁菌门相对丰度的差异变化。灌胃粪肠球菌DH9003前和灌胃28 d后,从小鼠粪便中共检出254种差异代谢物,其中124种显著上调,130种显著下调。基于KEGG通路富集分析发现显著性水平较高的代谢通路有ABC转运蛋白和蛋白消化吸收通路。结论:小鼠摄入粪肠球菌DH9003后可在其体内定植并对肠道菌群和代谢能力有一定影响。To explore the potential probiotic effect of an Enterocin Y3 producing strain of Enterococcus faecalis DH9003,about 15 healthy C57 male mice were intragastrically administered with sterile saline(control group,n=7)and E.faecalis DH9003(treatment group,n=8).After 4 weeks,the feces of mice were collected to determine the effect of E.faecalis DH9003 on intestinal flora modification and metabolic function of mice.The results showed that E.faecalis DH9003 were colonized in large quantities in mice within 7 days after intragastric administration.The number of the strain increased slowly and became stable at 7-28 days.By observing the pathological characteristics of the intestinal and liver tissue sections of mice,the intake of E.faecalis DH9003 had a certain improvement effect on the intestinal mucosa barrier of mice,indicating that the strain made the intestinal mucosa of mice denser and longer.Meanwhile,the strain showed no pernicious influence on the livers of mice.The intragastric administration of E.faecalis DH9003 led to the different diversity of the treated group,and became the dominant species among different groups.The supplement of E.faecalis DH9003 had a certain regulatory effect on the bacterial community structure modification in the intestinal tract of mice,and the modification at the phylum level was the differences of relative abundance between Bacteroidetes and Firmicutes.A total of 254 differential metabolic substances were detected between control and treatment group,and 124 differential metabolic substances were up-regulated and 130 differential onces in turn were down-regulated.The enrichment analysis of KEGG pathway showed that the metabolic pathways with high levels of visibility included ABC transporter proteins and protein digestion and absorption pathways.In summary,E.faecalis DH9003 could be colonized in vivo and effect on intestinal flora and metabolic ability of the host in some degree.

关 键 词：粪肠球菌 粪肠球菌素Y3 肠道菌群 代谢组学 组织切片 

分 类 号：TS201.3[轻工技术与工程—食品科学]