超高效液相色谱-三重四极杆质谱联用仪测定葡萄中的两种甜味剂  

Determination of 2 Sweeteners in Grape by UPLC-MS/MS

作　　者：曹佳 陈旭晋 周锋杰 陆宇阳 CAO Jia;CHEN Xujin;ZHOU Fengjie;LU Yuyang(Suzhou Agricultural Products Quality and Safety Monitoring Center,Suzhou 215000,China)

机构地区：[1]苏州市农产品质量安全监测中心,江苏苏州215000

出　　处：《现代食品》2024年第1期177-180,共4页Modern Food

摘　　要：建立一种使用超高效液相色谱-三重四极杆质谱联用仪对葡萄样品中的甜蜜素(环己基氨基磺酸钠)和纽甜等甜味剂进行定性分析和定量检测的方法。样品经提取后上机,采用多反应监测模式,负离子扫描。在1~50ng·mL^(-1)呈良好的线性关系,加标回收率为87.6%~96.8%,相对标准偏差≤5.8%。该方法具有前处理方便快捷、检测效率高、抗干扰能力强、准确度高、重复性好等特点,可作为新鲜葡萄样品中部分甜味剂的检测方法,也可为检测其他固体样品提供参考。Establishing a method for the qualitative analysis and quantitative detection of sweeteners such as sodium cyclamate and neotame in grape samples using a ultra high performance liquid chromatography triple quadrupole mass spectrometer system.The samples are prepared and injected into the instrument after extraction,utilizing a multiple reaction monitoring mode and negative ion scanning.There is a good linear relationship in 1~50 ng·mL^(-1),with a spike recovery rate of 87.6%to 96.8%and the relative standard deviation≤5.8%.This method is characterized by its simplicity in sample preparation,high detection efficiency,accuracy,and good repeatability.It can be used for the detection of certain sweeteners in fresh grape samples.

关 键 词：甜蜜素 纽甜 葡萄 超高效液相色谱-三重四极杆质谱联用仪 

分 类 号：S663.1[农业科学—果树学]