NLK通过调控paxillin促进膀胱癌细胞的增殖、迁移、侵袭及上皮间质转化  

NLK Promotes the Proliferation,Migration,Invasion,and EMT of Bladder Cancer Cells by Regulating Paxillin

作　　者：陆建军 余琴 陈利雄 Lu Jianjun;Yu Qin;Chen Lixiong(Department of Urology,the Thired People's Hospital,Yuhang District,Hangzhou,Zhejiang 311115,China;Department of Urology,Beilun District People's Hospital,Ningbo 315000,China)

机构地区：[1]杭州市余杭区第三人民医院泌尿外科,浙江杭州311115 [2]宁波市北仑区人民医院泌尿外科,浙江宁波315000

出　　处：《哈尔滨医药》2024年第1期1-7,共7页Harbin Medical Journal

基　　金：宁波市医学科技计划项目(2017A23)。

摘　　要：目的探讨Nemo样激酶(nemo-like kinase;NLK)及桩蛋白(paxillin)对膀胱癌细胞增殖、迁移、侵袭及上皮间质转化(epithelial mesenchymal transition,EMT)的作用和机制,为膀胱癌的基础研究及临床预防和治疗提供理论依据。方法生物信息学分析NLK在膀胱癌组织中的表达。EdU染色实验、克隆形成实验以及transwell实验检测膀胱癌细胞的增殖、迁移和侵袭。RNA pull down实验检测膀胱癌细胞中NLK与paxillin的互作关系。Western blot实验检测膀胱癌细胞中NLK、p-paxillin、及EMT相关蛋白的表达。结果NLK和p-paxillin在膀胱癌中高表达;NLK与paxillin具有相互作用关系;沉默NLK抑制了膀胱癌细胞的增殖、迁移、侵袭及EMT、并抑制p-paxillin的表达;而paxillin过表达则逆转了沉默NLK对膀胱癌细胞增殖、迁移、侵袭、EMT及p-paxillin表达的作用。结论NLK通过调控paxillin促进膀胱癌细胞的增殖、迁移、侵袭及EMT,为膀胱癌的基础研究及临床预防和治疗提供理论依据。Objective This research was intended to exploring the effect and mechanism of kinase(NLK)and paxillin on proliferation,migration,invasion,and epithelial mesenchymal transition(EMT)of bladder cancer cells,and providing theoretical basis for basic research,clinical prevention and treatment of bladder cancer.Methods The expression of NLK in bladder cancer tissue was analyzed by bioinformatics analysis.The proliferation,migration and invasion of bladder cancer cells were detected by EdU staining assay,clone formation assay,and transwell assay.RNA pull down assay was used to detect the interaction between NLK and paxillin in bladder cancer cells.Western blot assay was used to detect the expressions of NLK,p-paxillin,and EMT-related proteins in bladder cancer cells.Results NLK and p-paxillin were highly expressed in bladder cancer;NLK interacted with paxillin;NLK downregulation inhibited the proliferation,migration,invasion,EMT,and p-paxillin expression of bladder cancer cells;paxillin overexpression reversed the effect of silencing NLK on proliferation,migration,invasion,EMT,and p-paxillin expression in bladder cancer cells.Conclusion NLK promoted the proliferation,migration,invasion and EMT of bladder cancer cells by regulating paxillin,providing theoretical basis for basic research,clinical prevention and treatment of bladder cancer.

关 键 词：膀胱癌 NLK PAXILLIN EMT 

分 类 号：R734.2[医药卫生—肿瘤]