十二烷基苯磺酸钠对恩拉霉素发酵的影响  

Effect of sodium dodecylbenzene sulfonate on the fermentation of enramycin

作　　者：张瑶瑶 胡杜芬 江连清 陈璐 胡永良 ZHANG Yaoyao;HU Dufen;JIANG Lianqing;CHEN Lu;HU Yongliang(Zhejiang Hisun Pharmaceutical Co.,Ltd.,Taizhou 318000,China)

机构地区：[1]浙江海正药业股份有限公司,浙江台州318000

出　　处：《发酵科技通讯》2024年第1期28-32,共5页Bulletin of Fermentation Science and Technology

摘　　要：为了研究十二烷基苯磺酸钠对恩拉霉素发酵的影响,首先在恩拉霉素发酵培养基中添加不同量的十二烷基苯磺酸钠进行实验,发现加入适量的十二烷基苯磺酸钠能使发酵效价显著增长,在此基础上对加入时间点进行优化,确定最佳加入时间;其次在最优条件下对发酵过程中的糖耗速度、氨基氮和pH的变化进行了分析;最后研究了优化后的发酵周期。实验结果表明:十二烷基苯磺酸钠在恩拉霉素发酵中起到了重要作用,添加适量的十二烷基苯磺酸钠,能使恩拉霉素发酵效价达到8958μg/mL,效价提高了58%,发酵周期由240 h降至216 h,缩短了24 h,节省了能耗,提高了生产效率。In order to research the effect of sodium dodecylbenzene sulfonate on the fermentation of enramycin,different amounts of sodium dodecylbenzene sulfonate were first added into the fermentation medium of enramycin.It was found that adding proper amount of sodium dodecylbenzene sulfonate could significantly increase the fermentation potency.On this basis,the optimal adding time was determined.Secondly,the changes of sugar consumption rate,amino nitrogen and pH in the fermentation process were analyzed under the optimal conditions.Finally,the optimized fermentation cycle was studied.These experimental results demonstrated that sodium dodecylbenzene sulfonate played an crucial role in the fermentation of enramycin,adding proper amount of sodium dodecylbenzene sulfonate could increase the potency of enramycin up to 8958μg/mL,the potency increased 58%,the fermentation cycle was reduced from 240 hours to 216 hours,shortening 24 hours,which can lower energy consumption and effectively improve the production efficiency.

关 键 词：恩拉霉素 发酵 十二烷基苯磺酸钠 菌体 细胞膜 

分 类 号：Q815[生物学—生物工程]