顶空固相微萃取-气相色谱-质谱法测定香青兰挥发性成分  

Determination of Volatile Components in Dracocephalum moldavica by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

作　　者：李晶 丁祖英 古丽米热·努拉吉 黄超 冯彤彤 陈国通 LI Jing;DING Zu-ying;Gulimire·Nulaji(Xinjiang Academy of Analysis and Testing,Urumqi,Xinjiang 830011)

机构地区：[1]新疆维吾尔自治区分析测试研究院,新疆乌鲁木齐830011 [2]毕克气体仪器贸易(上海)有限公司,上海201203

出　　处：《安徽农业科学》2024年第5期204-207,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目(2022D01A113);新疆维吾尔自治区科技支疆项目(2020E02122);新疆维吾尔自治区天山创新团队计划项目(202110498)。

摘　　要：[目的]建立一种通过顶空固相微萃取-气相色谱-质谱联用技术方法快速测定香青兰挥发性成分的方法。[方法]采用该方法对香青兰挥发性成分进行测定分析,参照色谱峰和保留时间进行定性分析,并选用面积归一化法进行定量分析,计算出各成分的相对百分含量。[结果]共检测出59种挥发性成分,主要有醛类(59.50%)、酯类(13.61%)、烯烃类(10.99%)、酮类(8.13%)、醇类(4.82%)、杂环类(2.82%)、呋喃类(0.13%)。[结论]该方法的使用具有试样用量少,省去了样品前处理过程,可以防止有机试剂带入的杂质对分析结果造成干扰,减少对色谱柱及进样口的污染,分析过程中减少挥发性成分的损失等优点,可以有效地分离出样品中挥发性成分,适用于香青兰挥发性成分的检测。[Objective]To establish a rapid method for determining the volatile components of Dracocephalum moldavica by headspace solid phase microextraction-gas chromatography-mass spectrometry.[Method]This method was used to determine and analyze the volatile components of Dracocephalum moldavica.Qualitative analysis was conducted based on chromatographic peaks and retention time,and quantitative analysis was performed using area normalization method to calculate the relative percentage content of each component.[Result]A total of 59 volatile components were detected,including aldehydes(59.50%),esters(13.61%),olefins(10.99%),ketones(8.13%),alcohols(4.82%),heterocycles(2.82%)and furans(0.13%).[Conclusion]The use of this method has the advantages of low sample size,omitting the sample pre-treatment process,preventing impurities brought in by organic reagents from interfering with the analysis results,reducing pollution to the chromatographic column and sample inlet,and reducing the loss of volatile components during the analysis process.It can effectively separate volatile components in the sample and is suitable for the detection of volatile components in Dracocephalum moldavica.

关 键 词：香青兰 挥发性成分 顶空固相微萃取法 气相色谱-质谱联用法 

分 类 号：R284[医药卫生—中药学]