基于中心复合设计法优化虾青素纳米脂质载体制备工艺  

Optimization of preparation of astaxanthin nanolipid carrier based on central composite design method

作　　者：荆永康 张炜 高红 隋成博 乜世成 宋林 JING Yongkang;ZHANG Wei;GAO Hong;SUI Chengbo;NIE Shicheng;SONG Lin(College of Chemistry and Chemical Engineering,Qinghai Normal University,Xining 810008,China)

机构地区：[1]青海师范大学化学化工学院,西宁810008

出　　处：《中国油脂》2024年第1期71-78,共8页China Oils and Fats

基　　金：高原人工湿地生态平衡构建与应用示范(2021-SF-139)。

摘　　要：为提高虾青素的生物利用度,增加其稳定性,采用均质乳化-探头超声法制备虾青素纳米脂质载体(AST-NLC)。以粒径和包封率为指标,通过单因素试验和中心复合设计法优化AST-NLC的制备工艺,并对制备的AST-NLC进行X射线衍射(XRD)和傅里叶红外光谱(FT-IR)结构表征,以及4、25、50℃下的热稳定性测试。结果表明:制备AST-NLC的最佳工艺条件为甘油三硬脂酸酯与中链甘油三酯质量比1∶11(总质量0.99 g)、乳化剂用量(吐温-80+大豆卵磷脂,质量比1∶1)3.8%(以水相质量为基准)、油水比1∶34(体积比)、均质速度5000 r/min、超声时间2 min、虾青素添加量2.0 mg,在此条件下制备的AST-NLC粒径为(79.30±1.21)nm,多分散系数(PDI)为0.01±0.01,包封率为(81.36±1.84)%,Zeta电位为(-18.13±2.83)mV;结构表征结果表明,AST-NLC中的虾青素是以分子的状态包裹在脂质体中;热稳定性结果显示,4℃更有利于ASTNLC的保存。综上,成功制备了粒径小、分散均匀且包封率较高的AST-NLC。In order to improve the bioavailability and increase the stability of astaxanthin,astaxanthin nanolipid carrier(AST-NLC)was prepared by the homogeneous emulsification-probe ultrasonic method.The preparation process of AST-NLC was optimized by single factor experiment and central composite design method with particle size and encapsulation rate as indexes,and the AST-NLC prepared was structurally characterized by X-ray diffraction(XRD)and Fourier infrared spectroscopy(FT-IR)and tested for thermal stability at 4,25℃and 50℃.The results showed that the optimal process conditions for preparing AST-NLC were mass ratio of glycerol tristearate to medium chain triglyceride 1∶11(total mass 0.99 g),emulsifier dosage(Tween-80+soybean lecithin,mass ratio 1∶1)3.8%(based on water phase mass),oil-water volume ratio 1∶34,homogeneous speed 5000 r/min,ultrasonic time 2 min,astaxanthin dosage 2.0 mg.Under the optimal conditions,the AST-NLC was prepared with a particle size of(79.30±1.21)nm,a polymer dispersity index(PDI)of 0.01±0.01,an encapsulation rate of(81.36±1.84)%,and Zeta potential(-18.13±2.83)mV.The structural analysis revealed that astaxanthin in AST-NLC was encapsulated in liposomes in the state of molecules.The thermal stability results showed 4℃was more favorable for the preservation of AST-NLC.In conclusion,AST-NLC with small particle size,homogenous dispersion and high encapsulation rate was successfully prepared.

关 键 词：虾青素纳米脂质载体 中心复合设计法 热稳定性 

分 类 号：TS201.1[轻工技术与工程—食品科学] TS201.4[轻工技术与工程—食品科学与工程]