毛蜂窝菌菌油化学成分分析与DPPH自由基清除活性  

Chemical compositions analysis and DPPH radical scavenging activity of fungus oil of Hexagonia apiaria Pers.:Fr.

作　　者：彭毓敏 李皓 朱峰 冼建忠 PENG Yumin;LI Hao;ZHU Feng;XIAN Jianzhong(Drug Dispensing Center,Guangdong Traditional Chinese and Western Medicine Integrated Hospital,Foshan 528200,China;School of Environment and Chemical Engineering,Foshan University,Foshan 528000,China;Shunde Testing Base,Guangdong Testing Institute of Product Quality Supervision,Foshan 528300,China)

机构地区：[1]广东省中西医结合医院药物调配中心,广东佛山528200 [2]佛山科学技术学院环境与化学工程学院,广东佛山528000 [3]广东产品质量监督检验研究院顺德基地,广东佛山528300

出　　处：《佛山科学技术学院学报（自然科学版）》2024年第2期74-80,共7页Journal of Foshan University(Natural Science Edition)

基　　金：佛山市科技计划项目公共服务能力提升专项资助(2018AB003721);佛山市卫生和计划生育局医学科研课题资助(20190273)。

摘　　要：目的分析毛蜂窝菌菌油的化学成分并评价其1,1-二苯基-2-苦肼基(DPPH)自由基清除活性。方法以乙醚为提取剂,采用连续回流渗漉法提取毛蜂窝菌菌油,通过硅胶柱层析对菌油进行精制。毛蜂窝菌菌油化学成分经过气相色谱-质谱联用(GC-MS)技术分析,采用面积归一法测定菌油中各组分的相对含量,再用分光光度法评价其DPPH自由基清除活性。结果经GC-MS分析,毛蜂窝菌菌油中一共分离出了223个峰,鉴定出了44种化合物,占菌油的45.067%。相对含量最高的为(E,E)-亚油酸甲酯(5.362%),其次是甘油三亚油酸酯(4.147%),亚油酸甲酯(4.098%),相对含量均超过了4%。菌油DPPH自由基消除活性实验结果显示,一半DPPH自由基被清除时,菌油样品浓度为4.3540 mg/mL。结论毛蜂窝菌菌油的化学成分丰富多样,含有烷烃类化合物12种,脂类化合物12种,烯烃类化合物4种,酸类化合物5种,醇类化合物2种,醚类化合物3种,以及醛、胺等化合物。且该菌油具有有效的DPPH自由基清除活性。Objective To analysis the chemical compositions of the fungus oil of Hexagonia apiaria Pers.:Fr.and to evaluate its DPPH radical scavenging activity.Methods The fungus oil was extracted by continuous reflux infiltration method with ether as the extracting agent,and purified by silica gel column chromatography.The chemical compositions of the fungus oil was analyzed by gas chromatography-mass spectrometry(GC-MS),and to determine the relative content of each component in the oil,the area-based normalization method was applied.The DPPH radical scavenging activity was evaluated by spectrophotometry method.Results By GC-MS analysis,223 peaks were isolated from the fungus oil,and 44 peaks among them were identified,accounting for 45.067%of the fungus oil.The highest content was methyl(E,E)-9,12-octadecadienoate(5.362%),followed by trilinolein(4.147%),and methyl linoleate(4.098%),with relative content exceeding 4%.The results of DPPH radical scavenging assay showed that the value of the half-half scavenging concentration(EC50)was 4.3540 mg/mL.Conclusion The fungus oil is rich in chemical composition,including 12 alkanes,12 lipid compounds,4 olefins,5 acids,2 alcohols,3 ethers,and other compounds(aldehyde,amine,etc.).The fungus oil also has potent DPPH radical scavenging activity.

关 键 词：毛蜂窝菌 菌油 化学成分 DPPH自由基清除活性 气相色谱-质谱联用技术 

分 类 号：TS22[轻工技术与工程—粮食、油脂及植物蛋白工程]