β-胡萝卜素高效降解菌株的筛选鉴定及产香研究  

Screening Identification and Aroma Production of β-carotene Efficient Degrading Strains

作　　者：梁伟[1] 蔡联合[1] 苏赞[1] 陈义昌[1] 邹克兴[1] 王明月 王伊 LIANG Wei;CAI Lianhe;SU Zan;CHEN Yichang;ZOU Kexing;WANG Mingyue;WANG Yi(Guangxi Tobacco Industry Co.,Ltd.,Nanning,Guangxi 530001,China)

机构地区：[1]广西中烟工业有限责任公司,广西南宁530001 [2]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《农产品加工》2024年第4期20-24,共5页Farm Products Processing

基　　金：广西中烟工业有限责任公司科技计划项目“功能微生物提升真龙卷烟原料醇化质量技术研究”(GXZYCX2021B014)。

摘　　要：采用梯度稀释涂布法从常年落叶的土壤中筛选出3株具有降解β-胡萝卜素能力的菌株,通过分光光度法测定发酵液中β-胡萝卜素的降解率,进而对发酵液中的产物进行GC-MS分析,筛选出目标菌株TR-3,其降解β-胡萝卜素的能力在3株中最强,降解率达86.65%。革兰氏染色和光学显微镜观察结果表明,该菌株为革兰氏阴性杆菌;16S rDNA测序及聚类发育分析结果表明,菌株TR-3为肠杆菌属;气质联用(GC-MS)法分析发现,其对β-胡萝卜素降解产物中的成分主要为仲辛酮(37.69%)、苯乙醇(13.88%)、磷酸三丁脂(5.55%)、癸酸乙酯(5.54%)、β-紫罗兰酮(4.80%)及月桂酸乙酯(3.73%)等致香物质。In this experiment,three strains with the ability to degradeβ-carotene were preliminarily screened from the perennial deciduous soil by gradient dilution coating method.The degradation rate ofβ-carotene in the fermentation broth was determined by spectrophotometer.The products in the fermentation broth were analyzed by GC-MS,and the target strain TR-3 was screened out.The ability to degradeβ-carotene was the strongest among the three strains,and the degradation rate was 86.65%.The results of Gram staining and optical microscopy showed that the strain was Gram-negative bacilli.The results of 16S rDNA sequencing and cluster development analysis showed that strain TR-3 was Enterobacter.The degradation products were preliminarily analyzed by gas chromatography-mass spectrometry(GC-MS).The products were mainly aromatic substances such as 2-octanone(37.69%),phenylethyl alcohol(13.88%),tributyl phosphate(5.55%),ethyl decanoate(5.54%),β-ionone(4.80%)and ethyl laurate(3.73%).

关 键 词：Β-胡萝卜素 高效降解菌 筛选 分离纯化 致香物质 

分 类 号：Q939.99[生物学—微生物学]