固定化脂肪酶ANL-MARE催化酸解合成1-油酸-2-棕榈酸-3-亚油酸甘油三酯  

Synthesis of 1-Oleic Acid-2-Palmitic Acid-3-Linoleic Acid Triglyceride via Enzymatic Acidolysis by Immobilized Lipase ANL-MARE

作　　者：康梦 何家强 冯孔龙 黄早成 李一峰 苗建银[1] 符姜燕 朱泽榕 刘果[1] 曹庸[1] KANG Meng;HE Jiaqiang;FENG Konglong;HUANG Zaocheng;LI Yifeng;MIAO Jianyin;FU Jiangyan;ZHU Zerong;LIU Guo;CAO Yong(College of Food Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,Guangzhou 510642,China;Guangdong Huiertai Biotechnology Co.Ltd.,Guangzhou 510642,China;Guangdong Meiweixian Flavoring Foods Co.Ltd.,Zhongshan 528437,China)

机构地区：[1]华南农业大学食品学院,广东省功能食品活性物重点实验室,广东广州510642 [2]广东惠尔泰生物科技有限公司,广东广州510642 [3]广东美味鲜调味食品有限公司,广东中山528437

出　　处：《现代食品科技》2024年第2期109-119,共11页Modern Food Science and Technology

基　　金：广东省科技厅重点实验室项目-广东省功能食品活性物重点实验室(2018B030322010);广州市重点研发计划项目(SL2022B03J01001);中山市科技局项目-广式酱油风味控制与智能制造研究创新团队项目(CXTD2020006)。

摘　　要：1-油酸-2-棕榈酸-3-亚油酸甘油三酯(OPL)是人乳中主要的脂质组成,为满足婴幼儿配方食品母乳化需求,该试验探究了新型固定化脂肪酶ANL-MARE催化制备OPL的方法。试验成功制备和表征了固定化酶ANLMARE,并以ANL-MARE为生物催化剂,建立了一种用三棕榈酸甘油三酯(PPP)、油酸(OA)和亚油酸(LA)高效酶催化制备富含OPL结构脂的方法。经单因素和响应面试验优化,获得OPL最优合成工艺为:PPP与总脂肪酸的摩尔比1:14.27,OA与LA摩尔比为1:0.76,脂肪酶添加量12.70%,反应温度50℃,反应4 h,此条件下产物中OPL相对含量为47.93%,2位棕榈酸(sn-2 PA)占总棕榈酸(PA)的质量分数(sn-2 PA相对含量)为71.69%。此外,固定化酶ANL-MARE与商业脂肪酶相比,表现出较好的催化合成OPL的活性。综上所述,固定化酶ANL-MARE具有催化制备OPL结构脂的重大潜力,为人乳脂替代脂的高效制备提供了新策略和理论基础。The demand for infant formula similar to breast milk is growing.To address this,this study investigated the catalytic preparation of 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride(OPL),the main lipid component in human milk,using a new immobilized lipase,ANL-MARE.The immobilized lipase ANL-MARE was successfully prepared and characterized.Next,an efficient enzyme-catalyzed preparation of lipids rich in OPL structures from tripalmitin,oleic acid,and linoleic acid was established using ANL-MARE as the biocatalyst.After optimization via single-factor and response surface experiments,the optimal OPL synthesis process was obtained,with the following characteristics:a tripalmitin:total fatty acids molar ratio of 1:14.27;an oleic acid:linoleic acid molar ratio of 1:0.76;the addition of 12.70%ANL-MARE;a reaction temperature of 50℃,and a reaction time of 4 h.Under these conditions,the relative OPL content reached 47.93%,and sn-2 palmitic acid accounted for 71.69%of the total palmitic acid content(reflecting the relative sn-2 palmitic acid content).In addition,the immobilized lipase ANL-MARE showed better catalytic activity than commercial lipases in OPL synthesis.In conclusion,the immobilized lipase ANL-MARE has significant potential to catalyze the preparation of lipids with OPL structures.This study provides a novel strategical and theoretical basis for the efficient preparation of human milk fat substitutes.

关 键 词：1-油酸-2-棕榈酸-3-亚油酸甘油三酯 酶促酸解法 固定化脂肪酶 ANL-MARE 工艺优化 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]