基于Real-time PCR法检测乳粉中牛源性成分定量研究  

Quantitative Study on the Detection of Bovine Derived Components in Dairy Products Based on Real Time PCR Method

作　　者：陈晨 史国华 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍[1] CHEN Chen;SHI Guo-hua;CHEN Bo-xu;ZHANG Rui;WANG Yu-xin;JIA Wen-shen;CHEN Jia;ZHOU Wei(Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Hebei Food Inspection and Research Institute,Shijiazhuang,Hebei 050227,China;Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Chemical Technology,Shijiazhuang University,Shijiazhuang,Hebei 050035,China)

机构地区：[1]河北省食品安全重点实验室,国家市场监管重点实验室(特殊食品监管技术),特殊食品安全与健康河北省工程研究中心,河北省食品检验研究院,河北石家庄050227 [2]北京市农林科学院,北京100097 [3]石家庄学院化工学院,河北石家庄050035

出　　处：《粮油食品科技》2024年第2期159-164,共6页Science and Technology of Cereals,Oils and Foods

基　　金：河北省市场监督管理局科研计划项目(2022ZD11)。

摘　　要：基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。This experiment established a relative quantitative detection method for bovine derived components in milk powder based on Real time PCR.The specificity,sensitivity,and stability of cattle-specific primers and probes were tested.By simulating mixed samples of cow's milk powder and horse's milk powder with different concentrations,linear fitting was performed based on the functional relationship of their △Ct values,and a standard curve was drawn to establish a relative quantitative detection of bovine derived components in milk powder.The results showed that the minimum detection limit of this method was 0.000 01 mg/m L.The recovery rate was 91.11%~119.2%,with an inter group coefficient of variation of ≤0.58% and an intra group coefficient of variation of ≤1.44%.This method is suitable for detecting the adulteration of bovine derived components and content in milk powder in terms of specificity and stability.

关 键 词：牛乳粉 马乳粉 Real-time PCR 掺假检测 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]