Pharmacodynamic study of cannabidiol on bleomycin-induced pulmonary fibrosis in rats  

作　　者：SUN Meng-di ZHANG Fei-yu GAO Xin WANG Yu CHEN Ping-ping LIU Shu-min 

机构地区：[1]Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China [2]Institute of Traditional Chinese Medicine,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China

出　　处：《Journal of Hainan Medical University》2023年第22期1-8,共8页海南医学院学报（英文版）

基　　金：Post-doctoral Program of Heilongjiang Province (No.LBH-Z22251);Chinese Medicine Research Project of Heilongjiang Province (No.ZHY2022-114);Heilongjiang Chinese Medicine Association Youth Talent Promotion Project (No.2022-QNRC1-27)。

摘　　要：Objective:To study the protective effect of cannabidiol(CBD)on rats with pulmonary fibrosis and explore the possible mechanism of the use of CBD in the treatment of pulmonary fibrosis.Methods:Sixty SD rats were randomly divided into the normal control group,model group,prednisone group,CBD low,medium and high dose groups(12,36,108 mg/kg,ig),10 rats in each group.Except for the normal control group,the other 5 groups were all induced by tracheal injection of bleomycin to rat models of pulmonary fibrosis.After modeling,the rats were given intragastric administration once a day for 28 consecutive days and samples were taken.The degree of pulmonary edema was detected;the pathological changes of lung tissue were observed by HE and Masson staining;tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)and lung tissue superoxide dismutase(SOD),malondialdehyde(MDA),hydroxyproline(HYP)contents were measured by ELISA,transforming growth factor-β1(TGF-β1)andα-smooth muscle protein(α-SMA)concentration were detected by immunocytochemical method,real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect the mRNA expression levels of TGF-β1,α-SMA,Nrf2 and nuclear transcription factor-κB p65(NF-κB p65).Results:The lung organ coefficient and W/D value were significantly decreased in the CBD administration group(P<0.05);medium and high doses of CBD could reduce the number of collagen fibers and fibroblasts;the pulmonary fibrosis in the low,medium,and high dose groups of CBD was significantly lower.The levels of TNF-α,IL-1β,and IL-6 in rat serum,as well as MDA and HYP in lung tissue,were significantly lower compared to the model group.Additionally,the level of SOD was significantly increased(P<0.05);The expression ofα-SMA was decreased compared with the model group(P<0.05);the contents of TGF-β1,α-SMA and NF-κB p65 mRNA in lung tissue decreased,and the expression level of Nrf2 mRNA increased(P<0.05).Especially,the high-dose group had the most significant effect.Conclusion:CBD can sign

关 键 词：CANNABIDIOL Pulmonary fibrosis INFLAMMATION Oxidative stress PHARMACODYNAMICS 

分 类 号：R563[医药卫生—呼吸系统]