小麦肽通过抑制炎症和PI3K/mTOR通路改善乙醇诱导胃黏膜损伤  

Wheat peptides ameliorate ethanol-induced gastric mucosal damage by inhibiting inflammation and PI3K/mTOR pathway related proteins

作　　者：冯志远 张卓然 任杰 吴晗硕 秦慧民[1] 刘文颖[2] FENG Zhiyuan;ZHANG Zhuoran;REN Jie;WU Hanshuo;QIN Huimin;LIU Wenying(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Beijing Engineering Research Center of Protein&Functional Peptides,China National Research Institute of Food and Fermentation Industries,Beijing 100015,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《食品与发酵工业》2024年第6期92-99,共8页Food and Fermentation Industries

基　　金：北京市科技创新基地培育与发展工程专项(Z191100002819001);宁夏回族自治区重点研发计划项目(2021BEG02027)。

摘　　要：该研究的目的是探究小麦肽对乙醇造成的胃黏膜损伤的保护作用及其机制。使用小麦肽进行处理后,乙醇诱导小鼠胃黏膜损伤模型和人胃黏膜上皮细胞(human gastric mucosal epithelial cell,GES-1)细胞损伤模型,观察小鼠体重变化和胃组织损伤状况,评估氧化应激、炎症和自噬相关蛋白表达水平。小麦肽预处理显著改善了乙醇诱导的小鼠体重降低,提高了胃组织和GES-1细胞内抗氧化酶活力和一氧化氮(NO)含量,减少脂质过氧化发生。病理组织切片显示,小麦肽预处理后,胃组织损伤显著减少。此外,小麦肽降低了组织内促炎因子表达,并通过抑制GES-1细胞中PI3K/AKT/mTOR通路激活,提高了LC3-Ⅱ蛋白表达。小麦肽可以通过调节抗氧化能力和促炎因子表达,抑制PI3K/mTOR通路激活等方式,改善乙醇诱导的胃黏膜损伤。The objective of the study was to investigate the protective effect of wheat peptides on gastric mucosal injury caused by ethanol and its mechanism.After treatment with wheat peptides,ethanol induced gastric mucosal injury model in mice and human gastric mucosal epithelial cell(GES-1)injury model was used to observe the changes in body weight and gastric tissue damage and to assess the expression levels of oxidative stress,inflammation and autophagy-related proteins.Wheat peptides pretreatment significantly improved ethanol-induced weight loss in mice,increased antioxidant enzyme activity and nitric oxide(NO)content,and reduced lipid peroxidation in gastric tissues and GES-1 cells.Pathological tissue sections showed a significant reduction in gastric tissue damage after wheat peptides pretreatment.In addition,wheat peptides reduced tissue expression of pro-inflammatory factors and increased LC3-Ⅱprotein expression by inhibiting PI3K/AKT/mTOR pathway activation in GES-1 cells.Wheat peptides could improve ethanol-induced gastric mucosal injury by modulating antioxidant capacity,inhibiting pro-inflammatory factor expression and PI3K/mTOR pathway activation.

关 键 词：小麦肽 氧化应激 乙醇 人胃黏膜上皮细胞 MTOR信号通路 

分 类 号：TS201.4[轻工技术与工程—食品科学]