三种药用植物来源的蜂蜜抗氧化和抗菌活性研究  

Antioxidant and antimicrobial properties of three monofloral kinds of honey from medicinal plants

作　　者：张国志 韩凌云 李珊珊 郑火青[1] 胡福良[1] ZHANG Guozhi;HAN Lingyun;LI Shanshan;ZHENG Huoqing;HU Fuliang(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,浙江杭州310058

出　　处：《食品与发酵工业》2024年第6期100-108,共9页Food and Fermentation Industries

基　　金：国家蜂产业技术体系专项(CARS-44);2021浙江省农业重大技术协同推广项目(2021XTTGXM06)。

摘　　要：该文研究了3种药用植物来源的蜂蜜(茴香蜜、枸杞蜜和玄参蜜)的抗氧化能力和抗菌活性,并与麦卢卡蜜进行对比分析。用DPPH自由基、ABTS阳离子自由基清除能力和铁离子还原/抗氧化能力(ferric ion reducing/antioxidant power,FRAP)评价蜂蜜的抗氧化能力。琼脂扩散法(抑菌圈)和微量肉汤稀释法用于表征蜂蜜对革兰氏阳性菌(金黄色葡萄球菌和表皮葡萄球菌)、革兰氏阴性菌(大肠杆菌、鲍曼不动杆菌和铜绿假单胞菌)和真菌(白色念珠菌)的抗微生物能力。结果表明,麦卢卡蜜的总酚含量和抗氧化能力高于其他3种蜂蜜。蜂蜜的抗氧化能力和电导率、色值、总酚含量之间具有相关性(r≥0.727,P<0.01)。茴香蜜对金黄色葡萄球菌和表皮葡萄球菌的抗菌能力最强。茴香蜜和枸杞蜜对鲍曼不动杆菌和铜绿假单胞菌的抗菌能力高于麦卢卡蜜。麦卢卡蜜对大肠杆菌的抗菌活性高于采集的茴香蜜、枸杞蜜和玄参蜜。3种药用植物来源蜂蜜的抗菌活性与pH、淀粉酶活性和过氧化氢含量呈线性相关。The purpose of this study was to compare the antioxidant and antimicrobial activities of three monofloral kinds of honey from medicinal plants(fennel,wolfberry,and figwort)and to compare it with manuka honey.The antioxidant activities of honey samples were assessed by DPPH free radicals,ABTS cationic radicals scavenging activities,and ferric reducing/antioxidant power(FRAP)assay.The agar diffusion assay(zone of inhibition)and the broth micro-dilution assay were used to characterize honey samples against Gram-positive bacteria(Staphylococcus aureus and Staphylococcus epidermidis),Gram-negative bacteria(Escherichia coli,Acinetobacter baumannii and Pseudomonas aeruginosa),and fungi(Candida albicans).Obtained results demonstrated that the total phenolic content and antioxidant activity of manuka honey were higher than those of the other three kinds of honey.There was a correlation between the antioxidant activity of honey and electrical conductivity,color value,and total phenolic content(r≥0.727,P<0.01).Fennel honey had the strongest antibacterial activity against S.aureus and S.epidermidis.The antibacterial activities of fennel honey and wolfberry honey against A.baumannii and P.aeruginosa were higher than that of manuka honey.The antibacterial activity of manuka honey against E.coli was higher than fennel honey,wolfberry honey,and figwort honey.The antibacterial activities of the three monofloral kinds of honey from medicinal plants were linearly correlated with pH,diastase activity,and hydrogen peroxide content.

关 键 词：蜂蜜 抗氧化能力 抗菌活性 麦卢卡蜜 

分 类 号：S896.1[农业科学—特种经济动物饲养]