乳糖胆盐体系大肠菌群测试片的研制及性能验证  

Development and performance verification of a coliform test strip based on lactose bile salt system

作　　者：王杰伟 孙万东 吴艳辉 何艳玲 WANG Jiewei;SUN Wandong;WU Yanhui;HE Yanling(Beijing Landbridge Technology Co.Ltd.,Beijing 100123,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]北京陆桥技术股份有限公司,北京100123 [2]中国检验检疫科学研究院,北京100176

出　　处：《食品与发酵工业》2024年第6期267-273,共7页Food and Fermentation Industries

基　　金：中国检验检疫科学研究院基本科研业务费专项资金资助项目(2022JK29)。

摘　　要：该文以结晶紫中性红胆盐培养基(violet red bile agar,VRBA)组分为体系基础,通过筛选凝胶,正交试验优化凝胶、乳糖与胆盐添加量,以及添加乳糖发酵诱导剂,进行了大肠菌群测试片的研制。研究表明,使用卡拉胶代替琼脂作为载体,将卡拉胶、乳糖与胆盐的添加量分别优化为15、10、1.2 g/L,同时添加250 mg/L乳酰基-N脂酰基鞘氨醇作为乳糖发酵诱导剂,大肠菌群测试片的理化与微生物性能达到最优。测试片性能符合国标方法对VRBA培养基的控制要求;与国标大肠菌群平板计数方法相比,测试片方法在实际样本的检测结果无明显差异的同时,将检测周期缩短了24 h,结果观察更为直观,提升了对弱产气型目标菌的检测能力。该文所研制的大肠菌群测试片便捷快速准确,可适用于食品检测,并可为商业化测试片的研制提供借鉴意义。This study aimed to develop an accurate and rapid test strip for the detection of coliform bacteria in food samples,as a complementary solution to address the limitations of long detection cycles and low detection rates of weak-fermenting bacteria in national standard detection methods.Based on the composition of the violet red bile agar(VRBA),the coliform test strip was developed by screening different gelling agents,optimizing the concentration of gelling agent,lactose and bile salt using an orthogonal test,and including a lactose fermentation inducer.Results showed that the physicochemical and microbial properties of the coliform test strip reached the highest level when carrageenan was used instead of agar as the cold water soluble gel,carrageenan,lactose and bile salt were added at 15,10,and 1.2 g/L,respectively,and lactyl-N fatty acyl sphingosine was added at 250 mg/L as the lactose fermentation inducer.The performance of the test strips met the control requirements for VRBA culture medium using the national standard method.Compared with the national standard plate count method for coliform bacteria,the test strip method reduced the detection time by 24 hours without significant differences in the actual sample detection results.In addition,result interpretation with the test strip was more intuitive and the detection ability for weakly gas-producing target bacteria was improved.The coliform test strip developed in this study is easy to use,fast and accurate.It is suitable for food testing and can serve as a good reference for the development of commercial test strips.

关 键 词：大肠菌群 测试片 冷水可溶凝胶 诱导剂 

分 类 号：TS207.4[轻工技术与工程—食品科学]