高丁醇耐受性丙酮丁醇生产菌株选育研究进展  

Research progress in screening of acetone,butanol and ethanol producing strains with high butanol-tolerant

作　　者：丁欢欢 黄思怡 周秋香 卜京 施超越 李汉广[1] DING Huanhuan;HUANG Siyi;ZHOU Qiuxiang;BU Jing;SHI Chaoyue;LI Hanguang(Institute of Applied Microbiology/College of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;Affiliated Hospital of Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学生物科学与工程学院,应用微生物研究所,江西南昌330045 [2]江西农业大学附属医院,江西南昌330045

出　　处：《食品与发酵工业》2024年第6期300-306,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(21466014);江西省自然科学基金项目(20202BABL203042);江西省研究生创新专项资金项目(YC2022-S433,YC2021-S350)。

摘　　要：以化石燃料为主的传统不可再生能源在促进经济发展过程中起到重要作用,但同时也对生态环境造成了严重的危害,因此,新型可再生能源的研究与应用已成为全球能源领域重要方向。生物丁醇因具有环境友好、可再生等优点而成为生物质能源领域研究的热点。然而由于丁醇的毒害作用导致丙酮丁醇发酵过程中出现低产物浓度、低产率及低转化率现象,因此提高发酵菌株的丁醇耐受性是增强丙酮丁醇发酵经济性的重要途径之一。该文综述了不同诱变育种、适应性驯化策略、合成生物学手段等不同方法提高丁醇生产菌株的丁醇耐受性的原理及国内外研究进展,讨论了各方法在实践中的优缺点与挑战等问题,以期为提高微生物细胞的丁醇耐受性菌株的选育提供一些理论与实践参考。The fossil fuels as a mainly traditional non-renewable energy sources perform an important role in promoting economic development.However,a serious threat to the ecological environment occurred due to theirs application.Therefore,the research and application of new renewable energy has become an important direction in both industrial and academic worlds.Biobutanol has attracted renew interest in the field of biomass energy because of its environmental friendly and renewable advantages.However,butanol toxicity to the current producing microorganisms limits its accumulation in the fermentation broth,and for this reason the acetone,butanol,and ethanol(ABE)fermentation is of low production,yield and conversion efficiency.Therefore,improvement of butanol tolerance of butanol producing strains is one of the promising ways to enhance the economically competitive of ABE fermentation.This review summarized the principles of different methods,such as various mutagenesis breeding,adaptive domestication strategies,and synthetic biology,to improve butanol tolerance of butanol-producing strains.And we also reviewed its domestic and international research progress.Furthermore,we also discussed the challenges,advantages and disadvantages of those methods in the applications.The results from this review will provide some theoretical and practical references for screening of some higher butanol tolerance of microorganisms.

关 键 词：丙酮丁醇发酵 耐受菌株 菌种选育 基因工程 合成生物学 

分 类 号：TQ923[轻工技术与工程—发酵工程]